Cornell University
Engineered Oligosaccharyltransferases With Unique N-Glycosylation Site Preferences
Abstract
dc:description.abstractThe key enzyme in the Campylobacter jejuni glycosylation pathway, PglB, has been one of the well-studied asparagine-linked oligosaccharyltransferase (OST) in bacterial glycoengineering area. While C. jejuni PglB performs well in transferring different glycans, the acceptor sites it can recognize are limited. We provided a thorough investigation into Desulfovibrio desulfuricans PglB (DdPglB), an OST sharing homology with C. jejuni PglB but having more relaxed sequon specificity. The sequon logo of DdPglB against XXNXT library were built through a high-throughput assay. Two engineered OSTs combining distinct transmembrane domain and periplasmic domain of PglBs from C. jejuni and D. desulfuricans was also investigated. And the study interestingly revealed they can lose all their glycosylation activities after the combination. According to the sequon logo we built and further verification, a notable sequon QYNST, which is a native acceptor sequon of Fc domain of human immunoglobulin G, was able to be glycosylated by DdPglB. Overall, we presented a series of sequons that can be glycosylated by DdPglB and further expanded the glycoengineering toolbox with more potential acceptor sites that were not glycosylated before.
Degree
thesis:*- Name thesis:degree_name
- M.S., Chemical Engineering
- Level thesis:degree_level
- Master of Science
- Discipline thesis:degree_discipline
- Chemical Engineering
- Grantor
- Cornell University
- Year dc:date.issued
- 2019
Author and committee
dc:creator, dc:contributor.*- Author dc:creator
-
- Chen, Feng-Yang
- Committee members dc:contributor.committeemember
-
- Daniel, Susan
- Paszek, Matthew J.
Subjects
dc:subject × 8Rights
- Language dc:language.iso
- en_US
Identifiers
dc:identifier.*- Dc Identifier Other
-
ProQuest Submission ID: 10624
ProQuest Publication ID: 13904844 - OAI identifier oai:identifier
- oai:ecommons.cornell.edu:1813/67663