Global ETD Search
Search theses and dissertations gathered from participating repositories worldwide. Every result links back to the library that holds it. No account is needed.
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Showing 1 to 20 of 387 for “"Glycosylation"”.
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The glycosylation of Dichelobacter nodosus fimbriae
… serotypes A-NZ4, B₁-NZ2 and F-NZ3 have potential glycosylation sites. PAS staining to detect carbohydrate on SDS-PAGE gels indicated that some of D. nodosus fimbrial subunit proteins from serotypes A-NZ4, B₁-NZ2 and F-NZ3 may be glycosylated. Fourteen fimbrial subunit proteins previously stain …
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Congenital Disorder of Glycosylation (CDG) - Ih
Congenital Disorders of Glycosylation (CDG) comprise a rapidly growing group of multisystemic inherited disorders caused by mutations in genes which are required for the biosynthesis of glycoproteins. Here the molecular defect in a new type of CDG with an unusual clinical phenotype and a difficult …
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Expression and glycosylation of meprin isoforms
… Meprins were characterized with regard to their glycosylation by lectin blotting. Both meprin A and meprin B bound the lectins concanavalin A and the erythroagglutinin from <i>Phaseolus vulgaris</i> indicating that both enzymes contain high mannose and bisected biantennary complex type …
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Modeling congenital disorders of glycosylation in Caenorhabditis elegans: Genetic influences and structural consequences of N-linked glycosylation
… in all metazoans. This process, known as N-glycosylation, is complex and is achieved by the precise interactions of various cellular components. The initial stage of N-glycosylation occurs in the endolasmic reticulum and is preserved among eukaryotes. Glycans are further developed in the …
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Complex N-Glycosylation in Anti-Tumor Immunity
… anti-tumor target for two reasons: (i) protein glycosylation is known to play a role in tumor progression, and (ii) alternatively glycosylated proteins may function as tumor neoantigens. The glycosyltransferase Mgat5 catalyzes the formation of 1,6-N-acetylglucosamine branched glycans, and …
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Impaired N-Glycosylation Processing Impacts Neuronal Function
… are carried out primarily using N-linked glycosylation. N-glycosylation has the potential to co-translationally and post-translationally modify protein function by the addition and processing of oligosaccharides. These oligosaccharides are known as glycans and are broken up into three …
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Effects of Glycosylation on Lecithin -Cholesterol Acyltransferase
LCAT glycosylation mutants N84Q and N384Q were examined to determine the effects of deleting individual glycan chains. Purified N84Q LCAT did not possess measurable enzymatic activity or interfacial binding affinity for rHDL. Purified N384Q was more enzymatically active than WT LCAT, but lost all …
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N-Glycosylation Protects Host from Mycobacterial Infection
… screen, we have identified the early steps of N-glycosylation are required for the macrophage to resist mycobacterial infections. N-glycosylation attaches an oligosaccharide molecule to nascent secretory or membrane proteins within the endoplasmic reticulum (ER) for protein folding and …
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Protein expression and glycosylation in CHO cells
… depends on sufficient expression and correct glycosylation of the recombinant product. Low product titer and inconsistent protein glycosylation constitute two major problems frequently encountered in biopharmaceutical production. Using Chinese Hamster Ovary (CHO) cells expressing recombinant …
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REGULATION OF GLYCOSYLATION IN MAMMALIAN CELL LINES: INSIGHTS FROM STRUCTURAL GLYCOMICS, ANALYSIS OF GLYCOSYLATION-RELATED GENES AND FUNCTIONAL RNAI SCREENS
… to elucidate the regulation mechanism of glycosylation in mammalian cell lines. First, N-linked and O-linked glycan structures of five commonly used mammalian cell lines, namely CHO-K1, BHK-21, HEK293, COS-7 and 3T3, were characterized by a combination of mass spectrometric and …
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Omics-driven Insights into Congenital Disorders of Glycosylation
Contains fulltext : 327958.pdf (Publisher’s version ) (Open Access)
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Engineered Oligosaccharyltransferases With Unique N-Glycosylation Site Preferences
The key enzyme in the Campylobacter jejuni glycosylation pathway, PglB, has been one of the well-studied asparagine-linked oligosaccharyltransferase (OST) in bacterial glycoengineering area. While C. jejuni PglB performs well in transferring different glycans, the acceptor sites it can recognize …
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Investigating the trafficking of Golgi-resident glycosylation enzymes
… mediated by a large number of Golgi-resident glycosylation enzymes. These enzymes are largely type II transmembrane domain (TMD) proteins consisting of a short N-terminal cytosolic tail, a short TMD and a lumenal ‘stem’ region which separates the catalytic domain and the lipid bilayer. The …
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IS B4GALT5 DEFICIENCY A NEW CONGENITAL DISORDER OF GLYCOSYLATION?
… for two human congenital disorders of glycosylation (CDG) characterized by clinical pictures not really predicted by the corresponding mouse KO models. Previous studies showed that two similar galactosyltransferases, B4GALT5 and B4GALT6, are both responsible for the synthesis of …
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Sulfoxide Catalyzed Dehydrative Glycosylation. Reaction Development and Mechanistic Analysis
A sulfoxide-catalyzed dehydrative glycosylation for the coupling of carbohydrate hemiacetals and nucleophilic alcohols has been developed. In this procedure, a glycosyl hemiacetal is treated with the reagent combination of benzenesulfonic anhydride and a substoichiometric amount of n-butyl …
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Modulation of airway mucin glycosylation by Pseudomonas aeruginosa pyocyanin
… colonization of CF lungs by increasing the glycosylation of airway mucins with sialyl-Lewisx.
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Bacterial modulation of host glycosylation - in infection, biotechnology, and therapy
… aimed at exploring bacterial modulation of host glycosylation in infection and to evaluate the usefulness of bacterial enzymes in biotechnology and for therapeutic use. The role of IgG endoglycosidase EndoS in streptococcal virulence was evaluated in a murine model of invasive infection and we …
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Protein glycosylation in the gram-negative gamma proteobacterium photorhabdus luminescens
… glycoproteins and thus contains a protein glycosylation system. P. luminescens is a pathogen of insects and a symbiont of soil nematodes. Adhesion and invasion are very important in the life cycle of the organism and it is speculated that the bacteria may produce glycoproteins to facilitate …
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Investigating asparagine-linked protein glycosylation in eukaryotic and prokaryotic systems
N-linked protein glycosylation is characterized by the formation of a -glycosylamine linkage to an asparagine residue within the Asn-Xaa-Ser/Thr consensus sequence. This modification is found in organisms from eukaryotic, archaeal and bacterial domains and is implicated in numerous cellular …
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Priming and processing glycosyltransferases in bacterial N-linked glycosylation pathways
… chapter, the enzymes in the N-linked protein glycosylation pathway in Campylobacter jejuni were evaluated for their tolerance for azide-modified UDP-sugar substrates. In vitro experiments were employed to investigate the potential of these enzymes for executing the chemoenzymatic synthesis of …
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