The Effects Of Curcumin On The Expression Of P53 And Bcl-2 Oncoproteins On The Human Breast Carcinoma Cell Line MDA-MB 468

<p>We studied the effects of curcumin on the proliferation and viability of MDA-MB-468 breast cancer cells and its effects on the expression ofBcl-2 and p53 proteins in these cells. Based on previous studies, we predicted a dose-dependent increase in p53 (tumor suppressor) expression, and a decrease in Bcl-2 (anti-apoptotic oncoprotein) expression. Briefly, MDA-MB468 cells were seeded into 12-well plates at 2.0 * 104 cells/well and allowed to grow for 72 hrs. Cells were treated with curcumin at 0, 25, 50 and 100 pM in triplicate at time zero and cultured for another 24 hours. All data were obtained using a Muse Cell Analyzer. Proliferation and viability data were determined using an Annexin V and Dead Cell Kit, p53 expression was determined using a Multi-Colored DNA Damage Kit, and Bcl-2 expression was determined using a Bcl-2 Activation Dual Detection Kit. Results show a significant dose-dependent increase in both cell death and proliferation. However, there was no significant difference in expressions of both p53 and Bcl-2 oncoproteins across the four treatments. Future studies will investigate additional mechanisms by which curcumin induces these apoptotic effects.</p>

Name thesis:degree_name

Biology

Level thesis:degree_level

Thesis

Discipline thesis:degree_discipline

Biology

Year dc:date.available

2015

Author dc:creator

• Shajan, Angelin S.

Contributors dc:contributor

• Dr. Monica Frazier
• Dr. Kathleen Hughes
• Dr. Cindy Ticknor

• MDA-MB-468 Breast Cancer Cells
• P53
• Tumor Suppressor
• Bcl-2
• Biology
• Chemicals and Drugs
• Medical Biochemistry

Language dc:language

English

Repository record dc:identifier

https://csuepress.columbusstate.edu/theses_dissertations/180

OAI identifier oai:identifier

oai:csuepress.columbusstate.edu:theses_dissertations-1180