An enzymatic means for the rehabilitation of low-copy number and degraded DNA.

In spite of the immense success forensic DNA analysis has obtained over the last twenty-five years, a substantive challenge within the field of forensic DNA analysis is amplification and interpretation of degraded and low-copy number (LCN) DNA obtained from minimal and poor quality biological evidence. It has been well established that DNA profiles obtained from degraded samples are often of limited value due to the frequent occurrence of preferential amplification during polymerase chain reaction (PCR). The by-products of preferential PCR amplification are often observed as inter- and intra-locus peak imbalance, allelic dropout, and/or locus dropout. Inspired by advances in next-generation sequencing techniques, I propose a methodology for simultaneously normalizing the abundance of PCR products across all short tandem repeat (STR) loci using the DNA exonuclease, duplex-specific nucelase (DSN).

Grantor

Jackson College of Graduate Studies

Year dc:date.issued

2014

Author dc:creator

• Sambol, Nicole

Advisor dc:contributor.advisor

• Adams, Dwight

Committee members dc:contributor.committeemember

• Chooback, Lillian
• Creecy, James

Statement dc:rights

• All rights reserved by the author, who has granted UCO Chambers Library the non-exclusive right to share this material in its online repositories. Contact UCO Chambers Library's Digital Initiatives Working Group at diwg@uco.edu for the permission policy on the use, reproduction or distribution of this material.

Dc Identifier Other

(AlmaMMSId)9980658785202196

OAI record oai:identifier

https://shareok.org/server/oai/request?verb=GetRecord&metadataPrefix=dim&identifier=oai%3Ashareok.org%3A11244%2F325179