Global ETD Search
Search theses and dissertations gathered from participating repositories worldwide. Every result links back to the library that holds it. No account is needed.
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Showing 1 to 20 of 127 for “"DNA analysis"”.
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Mitochondrial Dna Analysis By Pyrosequencing
Mitochondrial DNA (deoxyribo nucleic acid) is typically used in forensic casework when small quantities of high molecular weight quality DNA is not expected to be present thus negating the chances of obtaining usable nuclear DNA. Typical samples that utilized mitochondrial DNA analysis are: hair, …
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Biochemical taxonomy by DNA analysis
… switched to use of differences at the level of DNA, since this is the most direct biochemical measure of genetic variation, being the material of which genes are made (Wilson et al., 1977). Organelle DNA from mitochondria in animals has been widely used since it has a number of advantages …
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Developing a Framework for Urban DNA Analysis
… the way they do? Is there an underlying urban DNA essence shaping their uniqueness? This research started with these fundamental questions and introduces the concept of urban DNA to explore them—not as just a metaphor but as a grounded framework that explains how cities evolve based on the …
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Passive micromixers for DNA analysis using CFD modelling
… is required between Deoxyribonucleic acid (DNA) sample and restriction enzyme for a fast digestion process. Mixing is improved by chaotic advection through serpentine mixing channels. This leads to the desired reduction in the fluid diffusion path while at the same time increasing the fluid …
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Simplified Low Copy Number Dna Analysis By Post Pcr Purification
… items contain an insufficient quantity of DNA to obtain complete or even partial DNA profiles using standard forensic gentotyping techniques. Here, various methods of post PCR purification were evaluated for their effects on the sensitivity of fluophore-based allelic detection. A method of …
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Evolution of asexuality in Daphnia pulex: Implications of mitochondrial-DNA analysis.
… electrophoresis and restriction fragment pattern analysis of mitochondrial DNA. A large amount of genetic variability was revealed by both the electrophoretic and mitochondrial data sets. A total of 104 genetically distinct clones were discovered. Comparison of the two data sets revealed that the …
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Phylogeographic and environmental DNA analysis of the mudpuppy (necturus maculosus maculosus)
… and evolutionary potential. Environmental DNA analysis complements traditional survey techniques and increases confidence when determining species’ range. Here, we explore the phylogeography of the mudpuppy’s western range and examine the feasibility of using eDNA to complement traditional …
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The development of PCR internal controls (PICs) for forensic DNA analysis
Proper interpretation of DNA profiles depends on the quality of the DNA samples, the amplification efficiency and the success of post-PCR processing. Chemicals associated with forensic samples can affect the amplification process while random errors occurring during pipetting and electrokinetic …
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DNA analysis of Ornithine Transcarbamylase (OTC) deficiency in South African patients
Hyperammonaemia is not an infrequent presentation in the newborn or neonatal period. While the majority are transitory in nature and due to infective processes or liver pathology/immaturity, a significant number are due to defects in enzymes of the urea cycle. This cycle has evolved to cope with …
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Genetic variation within the Icelandic goat breed: assessment using population data and DNA analysis
… on the base of both pedigree information and DNA analysis. The estimate is based on three approaches. Firstly, pedigree information is used to calculate the rate of inbreeding, pedigree completeness, generation interval, effective population size, highest contributing ancestors and …
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PROTON BEAM WRITING OF MICRO/NANO FLUIDIC DEVICES FOR DNA ANALYSIS AND PARTICLE SEPARATION
… devices were further used for the study of DNA configuration using a nanochannel confinement and particle separation using a ratchet structure. PBW is a direct write technique can be focused down to sub 30 nm size. Proton beam can penetrate a photoresist very deep with minimal proximity …
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Mitochondrial DNA analysis of the evolution and genetic diversity of ancient and extinct bears.
… is becoming more and more evident that ancient DNA studies greatly augment traditional mtDNA studies based only on extant lineages, and can reveal more complex scenarios than those hypothesised from modern data alone. Ancient DNA studies allow us to trace historic and ancient gene flow through …
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Sex estimation of unidentified human remains: Concordance between morphological anthropological assessment and DNA analysis
… but were biologically sexed male based on DNA analyses. This study aimed to assess the agreement of sexing methods in a Western Cape South African forensic sample of skeletonised individuals (n=126), who were morphologically estimated to have biological sex as female (n=41). Of the 41 …
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Bovine embryo microinjection, culture, microsurgery, and DNA analysis by the polymerase chain reaction technique
… of cellular material from bovine morulae for DNA amplification by the polymerase chain reaction technique (PCR), and evaluated the use of DNA microinjection, in vitro culture, morula bisection, and PCR for production and selection of transgenic bovine preimplantation embryos. In vivo …
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Analysis of mitochondrial DNA variation in the Egyptian population and its implications for forensic DNA analysis
The genetic sequence of human mitochondrial DNA (mtDNA) is of particular interest to forensic investigations involving human identification, as well as population genetics. The current mtDNA database is lacking sufficient representatives of African mitochondrial DNA sequences compared to European …
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The isolation of syaptic myonuclei and Schwann cell nuclei for DNA analysis from human skeletal muscle
The neuromuscular junction is a critical component of the motor system that enables transmission of impulses from the alpha-motor nerve to muscle fibers. Cerebral palsy is a neuromotor disorder that has been characterized by deficits in the central nervous system and, more recently, disruptions in …
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Investigating the Utility of Environmental DNA Analysis for the Monitoring and Management of Mid-Atlantic Alosine Fishes
Environmental DNA (eDNA) tools can address gaps in fish assessment data while reducing the cost and the impact of sampling on threatened anadromous alosine fishes in Chesapeake Bay. Here, I tested the ability of high-frequency eDNA sampling of river herring to predict fish abundances from …
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Algorithmic advances towards a fully automated DNA genotyping system
… (STR) genotyping is a leading tool in forensic DNA analysis. In STR genotyping, alleles in a sample are identified by measuring their lengths to form a genetic profile. Forming a genetic profile is time-consuming and labor-intensive. As the technology matures, increasing demand for improved …
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Assessment of the suitability of blood samples collected for toxicological analysis for subsequent genetic analysis: A follow-up study one year later
… sample from the deceased individual for genetic analysis. It was therefore important to determine the suitability of blood samples collected and handled in toxicology environments for subsequent genetic tests. Previously, blood samples from 30 post-mortem cases were collected into two red-top (no …
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Somatic Mutation Detection in Leukemia-Derived Circulating DNA: Utility in Monitoring Clonal Dynamics and Disease Response in Pediatric Acute Lymphoblastic Leukemia
… disease response in leukemia rely on the analysis of BM biopsy sample at specific time points throughout therapy. Not only the invasiveness of the BM biopsy hinders the sequential sampling, but also, the currently implied techniques are associated with a lack of sensitivity to detect …
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