Carleton University
Identification of Candidate Effector Proteins from Fusarium graminearum during Infection of Arabidopsis thaliana Using Proximity-Dependant Biotin Identification (BioID)
Abstract
dc:description.abstractFusarium graminearumis a fungal pathogen that causes Fusarium head blight (FHB) in cereal crops. Identifying proteins that are secreted from pathogens to overcome plant defenses and cause disease, collectively known as effectors, can reveal new targets for fungicides or other control measures.Proximity-dependent biotin identification (BioID) was used to identify potential effector proteins secretedin plantabyF. graminearumduring the infection ofArabidopsisseedlings. BioID analysis revealed over 300 proteins fromF. graminearum, of which 99 were considered to be candidate effector proteins (CEPs).A subset of CEPs was functionally characterized in wheat. Assays examining the ability of a CEP to induce cell death or affect the growth of a bacterial pathogen were performed to determine their role in plant defenses. The expression of four CEPs in wheat were found to alter bacterial growth, supporting their putative role as effector proteins promoting infection byF. graminearum.
Degree
thesis:*- Name thesis:degree_name
- Master of Science (M.Sc.)
- Level thesis:degree_level
- Master's
- Discipline thesis:degree_discipline
- Biology
- Grantor dc:publisher
- Carleton University
- Year dc:date.issued
- 2019
Author and committee
dc:creator, dc:contributor.*- Author dc:creator
-
- Miltenburg, Mary Gwen
Rights
dc:rights- Statement dc:rights
-
- Copyright © 2019 the author(s). Theses may be used for non-commercial research, educational, or related academic purposes only. Such uses include personal study, research, scholarship, and teaching. Theses may only be shared by linking to Carleton University Institutional Repository and no part may be used without proper attribution to the author. No part may be used for commercial purposes directly or indirectly via a for-profit platform; no adaptation or derivative works are permitted without consent from the copyright owner.
- Language dc:language.iso
- en
Identifiers
dc:identifier.*- OAI identifier oai:identifier
- oai:carleton.scholaris.ca:20.500.14718/39748