An experimental approach to determine the binding mode of yeast linker histone, Hho1p

In this study, we have developed an assay to investigate of the binding mode of yeast linker histone Hholp to nucleosomes, using magnetic bead pull-down assays. To understand the structural and regulatory role of, Hholp, the linker histone in Saccharomyces cerevisiae, and that of higher eukaryotes (Thoma et al., 1979), it is essential to determine the manner in which these histones associate with chromatin. It was previously proposed that Hh01p may bind to two nucleosomes simultaneously, where globular domain one and globular domain two bind to the adjacent nucleosomes.

Grantor dc:publisher.institution

Department of Molecular and Cell Biology

Year dc:date.issued

2003

Author dc:creator

• Smith, Elizabeth M

Advisor dc:contributor.advisor

• Patterton, Hugh

Language dc:language.iso

eng

Handle dc:identifier.uri

http://hdl.handle.net/11427/4333

OAI identifier oai:identifier

oai:open.uct.ac.za:11427/4333