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Search theses and dissertations gathered from participating repositories worldwide. Every result links back to the library that holds it. No account is needed.

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Showing 1 to 20 of 154 for “"Pull-down"”.

  1. Probing cellular protein complexes using single-molecule pull-down

    Jain, AnkurUniversity of Illinois at Urbana-Champaign2014

    … interaction between biomolecules is the pull-down assay. We have extended the conventional pull-down assay to its ultimate limit: the analysis of single biomolecular complexes. We achieve this goal by performing the pull-downs directly on to the surface of microscope slides and …

    uiuc Repository record

  2. Single molecule pull-down of viral RNA-protein complexes

    Srinivasan, DivyaUniversity of Illinois at Urbana-Champaign2012

    … regulation of mRNA. Recently, a single molecule pull-down (SiMPull) assay was developed to isolate and study single protein complexes directly from cell lysates. Unlike ensemble measurements, SiMPull is a powerful tool that allows detection of diverse proteins present in a single complex and …

    uiuc Repository record

  3. Single molecule pull down of double strand RNA binding proteins

    Wang, XinleiUniversity of Illinois at Urbana-Champaign2015

    … in mammalian cells. We applied single molecule pull-down (SiMPull) assay to investigate the intensity of various dsRNA-dsRBP interactions. Our results demonstrate that despite the highly conserved dsRNA binding domains, the dsRBPs exhibit diverse substrate specificy. While TRBP and ADAR1 have a …

    uiuc Repository record

  4. Characterisation of neurodegenerative diseases derived protein aggregates using improved single-molecule pull-down

    Zhang, YuUniversity of Cambridge2023

    … by establishing an improved single-molecule pull-down (SiMPull) assay, which was used to characterise soluble α-Synuclein (αS) and Amyloid-β (Aβ) aggregates in serum from patients with PD and controls. Our findings reveal that the ratio of detected aggregates (αS/(Aβ+αS)) and morphological …

    cambridge Repository record

  5. Structure and function of PhyA from Desulfovibrio magneticus and use of its product in affinity pull-down experiments

    Cleland, Colyn P., University of Lethbridge. Faculty of Arts and Science2019

    lethbridge

  6. A flotillin-1 és vimentin fehérjék kölcsönhatásának vizsgálata a PP2A Bα fehérjével endotél sejtekben

    Kaposi, Fanny2018

    … ismertettem. A fehérjéket termeltettük, majd pull down és Western blot kísérletekkel megvizsgáltuk. A kísérletek eredményét részletesen taglaltam, képekkel mellékelve.

    debrecen Repository record

  7. Structural and Biochemical Studies of the Innate Immune Pathway

    Lasker, Michael VirnelsonUniversity of Illinois at Urbana-Champaign2015

    … MyD88. In vitro transcription and translation pull-down experiments performed with IRAK-4 DD and MyD88 suggest that the ID of MyD88 might mediate this interaction.

    uiuc Repository record

  8. Distribution and targeting of CLC-3

    Zhifang ZhaoThe University of Texas Medical Branch2006

    … later times to localize in large vesicles. GST pull-down assays demonstrated that the N-terminal of ClC-3 binds to both clathrin and AP-2. Deletion of dileucine cluster within the cytosolic N terminal (amino acids 13-19) resulted in a molecule that had decreased endocytosis and increased surface …

    utmb Repository record

  9. Identification of novel components in fibroblast growth factor signalling

    Dudka, Anna AgnieszkaUniversity of Birmingham2010

    … signalling complexes and activation of downstream cascades. The goal of this project was to identify novel FGFR1 interacting proteins. The strategy employed for mapping novel partners on the basis of peptide-protein interaction was peptide pull-down. Using synthetic pairs of …

    birmingham Repository record

  10. An experimental approach to determine the binding mode of yeast linker histone, Hho1p

    Smith, Elizabeth MDepartment of Molecular and Cell Biology2003

    … Hholp to nucleosomes, using magnetic bead pull-down assays. To understand the structural and regulatory role of, Hholp, the linker histone in Saccharomyces cerevisiae, and that of higher eukaryotes (Thoma et al., 1979), it is essential to determine the manner in which these histones …

    cape-town Repository record

  11. Investigating Potential Multi-Protein Complexes Involving Emx2

    Enger, Nicole2019

    … a yeast two-hybrid screen and then confirmed by pull-down assays. The goal of this project is to use GST pull-down assays determine whether or not various combinations of these five proteins can interact to form a larger complex. The results indicate that larger protein complexes can be formed …

    central-wash Repository record

  12. Breaking the exocyst

    Sopipong, WatanyooUniversity of Dundee2020

    … variants and their partner exocyst subunits by pull-down assays. I used direct and inverted approaches, swapping the bait and prey, and also confirmed expression of both bait and prey by pull-down from the flow-through. The results suggest two exocyst breaker variants that prevent the …

    dundee Repository record

  13. Regulation of SNARE-dependent fusion in an in vitro system

    Aran-Ponte, VeronicaUniversity of Glasgow2009

    … SNARE proteins VAMP2 and syntaxin 4. The use of pull-down assays revealed the novel fact that Munc18c interacts not only with the t-SNARE syntaxin 4 but also with the v-SNARE VAMP2 via its SNARE motif. The SM:v-SNARE interaction was disrupted by the presence of syntaxin 4 revealing that these two …

    glasgow Repository record

  14. TRANSIENT PERFORMANCE EVALUATION OF AUTOMOTIVE SECONDARY LOOP SYSTEMS

    Eisele, Magnus2012

    … may increase power consumption and delay cool down while benefitting thermal comfort during start/stop operation. The recent revival of electric vehicles, as well as the associated focus on air-conditioning energy consumption, provides new challenges and opportunities. This research focuses on …

    maryland Repository record

  15. Screening auf Interaktionspartner für den hämatologischen Rezeptor PRV-1 : PRV-1 interagiert mit Decorin, C1qB und Defensin alpha 3

    Coch, Christoph

    … exprimiertem Protein durchgeführter GST Pull Down Assay keine konsistenten Ergebnisse lieferte, wurde ein bakterieller GST Pull Down Assay etabliert. Darüber konnte für die Proteine Decorin, C1qB und Defensin in vitro eine physikalische Interaktion mit PRV-1 nachgewiesen werden. Während …

    freiburg-diss Repository record

  16. The Contribution Of Respondent Computer Experience On Primacy Effect And Satisficing In Internet Surveys

    Cross, Freddie LeeVirginia Tech2005

    … listed visible vertical, visible horizontal, pull-down menu, or check-all-that-apply with two surveys for each formats; answer choices listed in forward order and answer choices listed in reverse) were used to test for primacy effect. Initial linear regressions revealed that 6 of the 85 …

    vt Repository record

  17. Viral subversion of host cell membrane trafficking

    Muenzner, JuliaUniversity of Cambridge2017

    … and NTD. Surprisingly, co-crystal structures and pull-down capture assays showed that cellular peptides containing clathrin-binding motifs can also bind multiple sites on the surface of NTD simultaneously. In addition, the structures of viral and cellular peptides bound to NTD enabled the …

    cambridge Repository record

  18. Evaluating protein-protein interactions of CpsA, a virulence factor in Group B Streptococcus

    Steffey, DanielleTexas Woman's University2019

    … between CpsA and CpsY may be transient. Our pull down did not find any of these proteins however, we have identified multiple additional proteins of interest, including FtsZ and CodY. Understanding protein-protein interactions of CpsA will help in new antimicrobial therapies targeting capsule …

    twu Repository record

  19. GENERATION OF FULL-LENGTH WILD-TYPE GATA4 PROTEIN AND CHARACTERIZATION OF ITS BINDING TO UNC-45 DOMAINS

    Anderson, Morgan2024

    … using protein-protein interaction techniques. Pull down assays suggested that GATA4 bound to the UCS domain of SM UNC-45, and possibly required flanking sequence in the central domain. Understanding this interaction can provide important mechanistic insight into how the SM UNC-45/GATA4 complex …

    sfasu Repository record

  20. Mycobacterium tuberculosis ClpC1: A potential target for tuberculosis drug discovery

    RIWANTO MELIANA2009

    … out to elucidate its mechanism of action. In a pull-down experiment with immobilized compound X and BCG lysate, ClpC was identified as the potential target. ClpC is an ATPase hexameric protein that catalyze the unfolding, disassembly and disaggregation of specific substrates in bacteria. ClpC …

    nus Repository record

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