Investigation of carbon catabolite repression in Clostridium beijerinckii NCIMB 8052

The substrate basis for the industrial acetone-butanol-ethanol (ABE) fermentations, has been agricultural products rich in starch or sucrose, and employed taxonomically distinct amylolytic and saccharolytic solventogenic clostridial strains respectively. There is evidence to suggest that the utilization of these substrates is subject to carbon catabolite repression. In Gram-positive bacteria, carbon catabolite repression is controlled by a global regulatory mechanism, central to which is an imperfect palindromic sequence, the cre element, which is recognized by a protein of the GalR-LacI family, the CcpA protein. A ccpA homologue, regA, has been previously identified in C. acetobutylicum NCP262 and successfully complemented a B. subtilis ccpA mutant strain. The sucrose operon from C. beijerinckii NCIMB 8052, scrARBK, has been characterised at the physiological and genetic levels with the ScrR repressor found to negatively auto-regulate the operon.

Grantor dc:publisher.institution

Department of Molecular and Cell Biology

Year dc:date.issued

2001

Author dc:creator

• Rafudeen, M S

Advisor dc:contributor.advisor

• Reid, Sharon J

Language dc:language.iso

eng

Handle dc:identifier.uri

http://hdl.handle.net/11427/4326

OAI identifier oai:identifier

oai:open.uct.ac.za:11427/4326