Genomic distribution of histone H1 in budding yeast (Saccharomyces cerevisiae) : yeast chromosome III

The linker histone HI binds to the nucleosome and is essential for the organization of nucleosomes into the 30-nm filament of the chromatin. This compaction of DNA has a well-characterized effect on DNA function. In Saccharomyces cerevisiae, HHO 1 encodes a putative linker histone with very significant homology to histone HI. In vitro chromatin assembly experiments with recombinant Hho 1 p have shown that it is able to complex with the dinucleosomes in a similar manner to histone HI. It has also been reported that disruption of HHOl has little affect on RNA levels. A longstanding issue concerns the location of Hho 1 p in the chromatin and studies have shown using immunoprecipitation technique with anti-HA antibody, that Hho 1 p shows a preferential binding to rDNA sequences. In this project we have tried to confirm the above results in wild type cells, using immunopurifi ed anti rHho 1 p antibody.

Grantor dc:publisher.institution

Department of Molecular and Cell Biology

Year dc:date.issued

2002

Author dc:creator

• Priya, Vattem Padma

Advisor dc:contributor.advisor

• Patterton, Hugh

Language dc:language.iso

eng

Handle dc:identifier.uri

http://hdl.handle.net/11427/4324

OAI identifier oai:identifier

oai:open.uct.ac.za:11427/4324