Global ETD Search
Search theses and dissertations gathered from participating repositories worldwide. Every result links back to the library that holds it. No account is needed.
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Showing 1 to 20 of 24 for “"rDNA sequences"”.
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The Isolation and Characterization of the Microbial Flora in the Alimentary Canal of <em>Gromphadorhina portentosa</em> Based on rDNA Sequences.
… members of the microflora based on ribosomal RNA sequences. Universal primers for Eubacterial, Archaeal, and Eukaryotic 16s/18s rRNA were then used for PCR amplification of total DNA isolated from gut contents. Sequences from isolates were compared using BLAST, ClustalW, and other programs to …
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Molecular phylogenetics of the Metazoan clade Lophotrochozoa
… major groups within the phylum Mollusca using sequences of the nuclear ribosomal large-subunit RNA gene (LSD rDNA). Results presented provide the first molecular evidence for a close relationship between the Scaphopoda and Cephalopoda. Phylogenetic trees with this topology were found to have …
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Genomic distribution of histone H1 in budding yeast (Saccharomyces cerevisiae) : yeast chromosome III
… that Hho 1 p shows a preferential binding to rDNA sequences. In this project we have tried to confirm the above results in wild type cells, using immunopurifi ed anti rHho 1 p antibody.
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Diversity, Distribution and Evolution of the Planktonic Diatom Family Chaetocerotaceae
… a particular marker sequenced, and the resulting sequences sorted into clusters or terminal clades assumed to represent species. Yet, reference barcodes of taxonomically validated species are needed to identify these clades. This exercise is the main aim of my thesis. <br></br><br></br> Since it …
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Comparative genomics of repetitive elements between maize inbred lines B73 and Mo17
… system, we analyzed whole genome shotgun (WGS) sequences for the two maize inbred lines B73 and Mo17 using k-mer analysis to quantify the differences between the two genomes. Significant differences were identified in highly repetitive sequences, including centromere, 45S ribosomal DNA (rDNA), …
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Variations of Vibrio Populations in Pacific White Shrimp, <i>Litopenaeus vannamei</i>, Aquaculture Raceways
… chain reaction) using primers targeting 16s rDNA sequences for total bacteria, the <em>Vibrio</em> genus, and <em>Vibrio parahaemolyticus</em> in aquaculture tanks over the growing season. Total DNA concentrations in each sample were measured with a NanoDrop 2000 Spectrophotometer (Thermo …
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Comparative Serological and Molecular Analysis of Group VIII <em>Spiroplasma</em> from Australia and North America
… by serology. Recent work has generated 16S rDNA sequences that generally correlate with the serological findings. Although the serology and the 16S rDNA sequence analysis clearly classifies strains to the group level, they do not distinguish between strains within the same group. The goal of …
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Detection of periodontal bacteria in atherosclerotic plaque
… identification of a diverse collection of 16S rDNA sequences within atherosclerotic plaque samples. Thirteen of 21 (61.9%) plaques contained bacterial 16S rDNA relating to periodontal (P. gingivalis and T. forsythia), oral (Streptococcus spp.), and respiratory (Klebsiella pneumoniae and …
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Generic concepts in the Crepidotaceae as inferred from nuclear large subunit ribosomal DNA sequences, morphology, and basidiospore dormancy patterns
… using nuclear large subunit ribosomal DNA (LSU rDNA) sequences. Parsimony analysis of the molecular data supports the Singer classification of Crepidotus, Melanomphalia, and Simocybe as a single monophyletic unit within the Agaricales. The affinities of the genus Tubaria remain uncertain. …
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Molecular Identification of Plant Pathogens Affecting Cannabis sativa Crops in Nevada
… DNA barcoding, to identify species based on rDNA sequences and analyzing viral capsid protein gene sequences, which enabled the successful identification of every encountered organism to a species level. The pathogens identified in this study include five Fusarium species: F. oxysporum, F. …
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Assessment of flagellate diversity at deep-sea hydrothermal vents using the combined approach of culture-dependent and culture-independent methods
… Choanoflagellates (the Opisthokonta). Using l8S rDNA sequences from most of the major eukaryotic lineages, maximum likelihood, minimum evolution and maximum parsimony analyses yielded congruent phylogenies supporting this hypothesis. Deep-sea vent samples were both cultured to select for …
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Tick-borne haemoparasite occurrence and Anaplasma bovis strain diversity in eastern rock sengis (Elephantulus myurus)
… Alignment of the near full-length 16S rRNA gene sequences of the A. bovis-like strain previously identified in rock sengi (Harrison et al., 2013) revealed that the A. bovis RLB probe differed at three nucleotide sites under the probe area explaining why the species-specific A. bovis RLB probe …
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Taxonomic classification of metagenomic sequences
… characterization of metagenomic shotgun sequences. However, the taxonomic classification of metagenomic sequences from novel species without close homologues in the biological sequence databases poses a challenge due to the high number of wrong taxonomic predictions on lower taxonomic …
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Mating interactions of the harmful dinoflagellate alexandrium tamarense from UK coastal waters
… Group I and Group III isolates may have rDNA pseudogenes corresponding to different ribotypes. If correct this could have implications for the overestimation of A. tamarense group diversity in natural populations when using rDNA sequences for identification.
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Psychrophilic and psychrotrophic Clostridium spp. associated with meat spoilage
… Although RFLP analysis of the amplified 16S rDNA gene allowed differentiation of psychrophilic and psychrotrophic clostridia at the genotypic species level and below, comparison of PCR-RFLP patterns and 16S rDNA sequences of meat isolates with patterns and sequences of reference strains did …
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Analysis of anther-derived plants of Solanum phureja: variation in ploidy, photosynthetic efficiency and structure of the nuclear genome
… replication resulting in an increase of sequences consisting of highly· repetitive DNA. Free solution RNA-DNA hybridization showed that the monoploid DNA contained 30% more rDNA sequences than the diploid. Southern blot analysis using rRNA as the probe revealed variation for copy number …
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Characterization of Extremophilic Bacteria for Potential in the Biofuel and Bioprocess Industries
… platform. Phylogenetic analysis of partial 16S rDNA sequences placed 1,200 of these isolates in the genus Bacillus. Other genera include, but are not limited to: Ureibacillus, Bacillus, Geobacillus, and Caldalkalibacillus. The central hypothesis of this dissertation was that selection due to the …
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Molecular and Field Analyses of Bathyplectes spp. (Hymenoptera: Ichneumonidae) in Alfalfa Systems in Virginia
… analyses were conducted to 1) derive genetic sequences for B. anurus and B. curculionis, which could be used to identify and differentiate the two species, and 2) test for genetic variation within and between regional populations of Bathyplectes. Sequences for each species were obtained for …
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Systematics and Ecology of Truffles (Tuber)
… 4 is a meta-analysis of all known Tuber ITS rDNA sequences (e.g. from Genbank and generated from herbarium collections) available at the time. These were placed within the Tuber phylogeny to assess species diversity, long-distance dispersal, and host associations. In total, 120 phylotypes …
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