The characterization of XVSAP1: a gene isolated from the resurrection plant Xerophyta viscosa Baker, and its expression in transgenic plants

The strategy of "complementation by functional sufficiency" was used to isolate a cDNA, designated XVSAP1, from a cDNA library constructed from dehydrated Xerophyta viscosa Baker leaves. Analysis of the cDNA sequence indicated a highly hydrophobic protein with six transmembrane regions. Southern blot analysis revealed that there are at least two copies of XVSAP1 in X. viscosa. The deduced amino acid sequence showed 49% identity to WCOR413, a low temperature-regulated protein from wheat. The protein also showed between 25% to 56% identity to WCOR413-like proteins from Arabidopsis thaliana.

Grantor

Department of Molecular and Cell Biology

Year dc:date.issued

2003

Author dc:creator

• Garwe, Dahlia

Advisors dc:contributor.advisor

• Thomson, Jennifer
• Mundree, Sagadevan

• Molecular and Cell Biology

Handle dc:identifier.uri

http://hdl.handle.net/11427/40101

OAI identifier oai:identifier

oai:open.uct.ac.za:11427/40101