Global ETD Search
Search theses and dissertations gathered from participating repositories worldwide. Every result links back to the library that holds it. No account is needed.
Results
Showing 1 to 20 of 153 for “"cDNA library"”.
-
Generation of A L. Hesperus embryonic cDNA library for the isolation of genes involved in early pattern formation
… spider, Latrodectus hesperus, an embryonic eDNA library and a fixation protocol were developed for L. hesperus embryos. The generation of these tools will allow comprehensive analysis of black widow spider development and give insight into whether, and how, spiders use Hox and Pax genes to …
-
Complementation of the salt sensitive S. cerevisiae mutant cch1 with an A. thaliana cDNA library and analysis of isolated genes
… phenotype. For screening an Arabidopsis cDNA library selection conditions were established. On the optimized media it was possible to distinguish between the growth rates of the wild type, transformed with a control plasmid, and the knock-out mutant, transformed with a control plasmid, …
-
Tsyn-Seq: A T Cell Synapse-Based Antigen Identification Platform
… functionally screen for target antigens from a cDNA library, productively interacting T cell–APC aggregates were detected by dual reporter activity and enriched by flow sorting followed by antigen identification quantified by deep sequencing (Tsyn-seq). When applied to a previously characterized …
-
Molecular cloning, immunolocalization, and transgenic expression in yeast and tobacco of three cDNAs that encode sugar transporters in sugar beet
… of a yeast mutant with a sugar beet cDNA library constructed in a yeast expression vector. The other approach was PCR amplification based on sequence homologies found in a superfamily of sugar transporters. No promising clones were isolated by functional complementation. In contrast, …
-
MOLECULAR ANALYSIS OF FLORAL PIGMENTATION IN THE ORCHID, BROMHEADIA FINLAYSONIANA
… involved the isolation and characterisation of cDNAs in the flavonoid biosynthetic pathway of a local species of orchid, Bromheadia finlaysoniana (Lindl.) Rchb. F. A cDNA library was constructed from the fully-opened flowers of Bromheadia finlaysoniana. cDNA clones encoding phenylalanine …
-
The plasma protein fetuin: common structural features of the mammalian fetuin family
… and were used to screen an adult bovine liver cDNA library by plaque hybridisation. A cDNA encoding the whole of the plasma protein was obtained and was fully sequenced on both strands. The deduced amino acid sequence was confirmed by protein sequence data in the literature [Christie et al., …
-
Identification and Function of Beta-1,4-Galactosyltransferase Associated Proteins
In summary, a pig testis phage display cDNA library screening and protein affinity chromatography were applied to identify GalTase-I associated proteins. Several proteins were identified as candidates that interact with GalTase-I and potentially play important roles during fertilization. …
-
The Production and Characterization of Xenopus Albumin Complementary-Dna Clones and Regulation of Rna Levels by Estrogen and Dexamethasone
… albumin by estrogen and dexamethasone, albumin cDNA clones were isolated from an 18S cDNA library produced from uninduced RNA. Several albumin clones were identified and characterized with respect to their restriction maps, and homology to each other. Clones corresponding to both 74,000 and …
-
Identification and Classification of Novel Gene Products Involved in Development and Regeneration in Xenopus Laevis
A cornea-lens transdifferentiation-specific cDNA library has been used to characterize new molecular events in lens regeneration. Most of these genes are also expressed during lens development, illustrating the similarity between mechanisms of development and regeneration. A subset of genes; whose …
-
The characterization of XVSAP1: a gene isolated from the resurrection plant Xerophyta viscosa Baker, and its expression in transgenic plants
… by functional sufficiency" was used to isolate a cDNA, designated XVSAP1, from a cDNA library constructed from dehydrated Xerophyta viscosa Baker leaves. Analysis of the cDNA sequence indicated a highly hydrophobic protein with six transmembrane regions. Southern blot analysis revealed that there …
-
Molecular characterization of XvPer1, a novel antioxidant enzyme from the resurrection plant Xerophyta viscosa, and AC3, a LEA-like protein from Arabidopsis thaliana
By differential screening of a cDNA library of the resurrection plant Xerophyta viscosa, a cDNA termed XvPer I was isolated for its over-expression under dehydration stress. Analysis of the cDNA sequence indicated a hydrophilic protein of a predicted Mw of 24.2 kDa with high identity to plant l-Cys …
-
Amino Acid Transporters in Arabidopsis Thaliana
… ART1, were identified from the Arabidopsis EST cDNA library by complementation. LHT1 is a lysine and histidine transporter, and ART1 is an arginine and aromatic amino acid transporter. ART1 seemed to possess transport activity for IAA and other aromatic amino acid derivatives. Two T-DNA tagged …
-
Study of the HIV-1 Gene Regulatory Proteins TAT and REV by the Two-hybrid System in Yeast
… large-scale screening of the members of a cDNA library for interaction with a protein of interest by the functional reconstitution of the GAL4 transcriptional activator.</p> <p>The search for Rev-interacting proteins from a mouse embryo cDNA library was conducted with a one-reporter-gene …
-
Bioinformatic and molecular approaches for the analysis of the retinal pigment epithelium (RPE) transcriptome
… in AMD pathogenesis. Towards this end, a bovine cDNA library enriched for RPE transcripts was constructed in-house using a PCR-based suppression subtractive hybridization (SSH) technique (Diatchenko et al., 1996, 1999), which normalizes for sequence abundance and achieves high enrichment for …
-
Tobacco Phospholipase D β1: Molecular Cloning and Biochemical Characterization
… was identified. A PLD clone was isolated from a cDNA library using the partial PLD as a probe: Nt10B1 shares 92% identity with PLDβ1 from tomato but lacks the C2 domain. PCR analysis confirmed insertion of the antisense fragment into the plants: three introns distinguished the endogenous gene …
-
Towards a comprehensive description of the human retinal transcriptome: identification and characterization of differentially expressed genes
… suppression subtracted hybridization (SSH) cDNA library. In total, 6603 EST clusters were evaluated during this thesis and detailed in-silico analysis was performed on 750 EST clusters. The expression of the genes was evaluated using reverse transcriptase-polymerase chain reaction (RT-PCR), …
-
Isolation And Characterization Of Human Ribosomal Protein Complementary-dnas
… experiments designed to identify genomic or cDNA clones that encode human ribosomal proteins. Two methods were employed to achieve the goal of obtaining full-length clones that would facilitate expression experiments in the future. The first, cross hybridization, used a ribosomal protein gene …
-
Chemoreceptors From the Hawk Moth Manduca Sexta L
… Differential screening of a male antennal cDNA library before the publication of the Drosophila melanogaster genome yielded one G coupled-protein receptor that had no matches in Genbank databases and the results of RT-PCR and quantitative real-time PCR experiments show MsOr1 is expressed …
-
Isolation and Characterization of a Novel Gene, xMADML, Involved in Xenopus Laevis Eye Development Using Cornea -Lens Regeneration
One clone was isolated from the cDNA library on the basis of its embryonic expression pattern and its sequence similarity to human NRBP and mouse MADM genes. This gene, xMADML, was characterized through molecular analysis, which showed both similarity and important differences to MADM, and protein …
Page 1 of 8