The primary structure of histone H2B from the mollusc Patella granatina

Histones H2B were isolated from the gonads of a mollusc (Patella granatina) and from chicken (Gallus domesticus), crocodile (Crocodylus niloticus) and amphibian (Xenopus laevis) erythrocytes. The H2B's were purified by ion-exchange and gel exclusion chromatography. The complete primary structure of the mollusc hi stone H2B t 11 has been deduced from the sequences established of pa e a adjoining and overlapping peptides by the Edman degradation procedure. The partial structure of H2B from chicken erythrocytes (87 residues), crocodile erythrocytes (75 residues) and Xenopus erythrocytes (63 residues) was also established. The amino acid sequences are compared to those of other histones H2B. The effect of mutations on the predicted secondary structure of histone H2B is considered.

Grantor

Department of Molecular and Cell Biology

Year dc:date.issued

1978

Author dc:creator

• Van Helden, Paul David

Advisor dc:contributor.advisor

• Von Holt, Claus

• Biochemistry

Handle dc:identifier.uri

http://hdl.handle.net/11427/38915

OAI identifier oai:identifier

oai:open.uct.ac.za:11427/38915