{"id":{"repo_id":"cape-town","oai_identifier":"oai:open.uct.ac.za:11427/38915"},"canonical_url":"https://search.dev.ndltd.org/etd/cape-town/oai:open.uct.ac.za:11427/38915","repository":{"repo_id":"cape-town","name":"University of Cape Town","base_url":"https://open.uct.ac.za/oai/request"},"display":{"title":"The primary structure of histone H2B from the mollusc Patella granatina","abstract":"Histones H2B were isolated from the gonads of a mollusc (Patella granatina) and from chicken (Gallus domesticus), crocodile (Crocodylus niloticus) and amphibian (Xenopus laevis) erythrocytes. The H2B's were purified by ion-exchange and gel exclusion chromatography. The complete primary structure of the mollusc hi stone H2B t 11 has been deduced from the sequences established of pa e a adjoining and overlapping peptides by the Edman degradation procedure. The partial structure of H2B from chicken erythrocytes (87 residues), crocodile erythrocytes (75 residues) and Xenopus erythrocytes (63 residues) was also established. The amino acid sequences are compared to those of other histones H2B. The effect of mutations on the predicted secondary structure of histone H2B is considered.","abstract_html":"Histones H2B were isolated from the gonads of a mollusc (Patella granatina) and from chicken (Gallus domesticus), crocodile (Crocodylus niloticus) and amphibian (Xenopus laevis) erythrocytes. The H2B's were purified by ion-exchange and gel exclusion chromatography. The complete primary structure of the mollusc hi stone H2B t 11 has been deduced from the sequences established of pa e a adjoining and overlapping peptides by the Edman degradation procedure. The partial structure of H2B from chicken erythrocytes (87 residues), crocodile erythrocytes (75 residues) and Xenopus erythrocytes (63 residues) was also established. The amino acid sequences are compared to those of other histones H2B. The effect of mutations on the predicted secondary structure of histone H2B is considered.","abstract_has_math":false,"creators":["Van Helden, Paul David"],"institution":"Department of Molecular and Cell Biology","degree_name":null,"degree_level":null,"degree_discipline":null,"degree_department":null,"school":null,"contributors":[],"advisors":["Von Holt, Claus"],"committee_chairs":[],"committee_members":[],"year":1978,"date_issued":"1978","date_published":"1978","updated_at":"2026-07-22T22:23:09Z","subjects":["Biochemistry"],"languages":[],"rights":[],"rights_urls":[],"identifier_entries":[]},"links":{"outbound_url":"http://hdl.handle.net/11427/38915","outbound_label":"Handle","outbound_source":"dc:identifier.uri"},"metadata_groups":[{"id":"people","label":"People","entries":[{"key":"dc:contributor.advisor","label":"Advisor","values":["Von Holt, Claus"]},{"key":"dc:creator","label":"Author","values":["Van Helden, Paul David"]}]},{"id":"academic_context","label":"Academic Context","entries":[{"key":"dc:date.accessioned","label":"Dc Date Accessioned","values":["2023-09-27T14:06:04Z"]},{"key":"dc:date.available","label":"Dc Date Available","values":["2023-09-27T14:06:04Z"]},{"key":"dc:date.issued","label":"Date","values":["1978"]},{"key":"dc:publisher.department","label":"Dc Publisher Department","values":["Department of Molecular and Cell Biology"]},{"key":"dc:type","label":"Dc Type","values":["Doctoral Thesis"]},{"key":"dc:type.qualificationlevel","label":"Dc Type Qualificationlevel","values":["Doctoral","PhD"]}]},{"id":"subjects_keywords","label":"Subjects and Keywords","entries":[{"key":"dc:subject","label":"Dc Subject","values":["Biochemistry"]}]},{"id":"identifiers","label":"Identifiers","entries":[{"key":"dc:identifier.uri","label":"Identifier URI","values":["http://hdl.handle.net/11427/38915"]}]},{"id":"additional","label":"Additional Metadata","entries":[{"key":"dc:description.abstract","label":"Abstract","values":["Histones H2B were isolated from the gonads of a mollusc (Patella granatina) and from chicken (Gallus domesticus), crocodile (Crocodylus niloticus) and amphibian (Xenopus laevis) erythrocytes. The H2B's were purified by ion-exchange and gel exclusion chromatography. The complete primary structure of the mollusc hi stone H2B t 11 has been deduced from the sequences established of pa e a adjoining and overlapping peptides by the Edman degradation procedure. The partial structure of H2B from chicken erythrocytes (87 residues), crocodile erythrocytes (75 residues) and Xenopus erythrocytes (63 residues) was also established. The amino acid sequences are compared to those of other histones H2B. The effect of mutations on the predicted secondary structure of histone H2B is considered."]},{"key":"dc:title","label":"Title","values":["The primary structure of histone H2B from the mollusc Patella granatina"]}]}],"canonical_facts":{"dc:contributor.advisor":["Von Holt, Claus"],"dc:creator":["Van Helden, Paul David"],"dc:date.accessioned":["2023-09-27T14:06:04Z"],"dc:date.available":["2023-09-27T14:06:04Z"],"dc:date.issued":["1978"],"dc:description.abstract":["Histones H2B were isolated from the gonads of a mollusc (Patella granatina) and from chicken (Gallus domesticus), crocodile (Crocodylus niloticus) and amphibian (Xenopus laevis) erythrocytes. The H2B's were purified by ion-exchange and gel exclusion chromatography. The complete primary structure of the mollusc hi stone H2B t 11 has been deduced from the sequences established of pa e a adjoining and overlapping peptides by the Edman degradation procedure. The partial structure of H2B from chicken erythrocytes (87 residues), crocodile erythrocytes (75 residues) and Xenopus erythrocytes (63 residues) was also established. The amino acid sequences are compared to those of other histones H2B. The effect of mutations on the predicted secondary structure of histone H2B is considered."],"dc:identifier.uri":["http://hdl.handle.net/11427/38915"],"dc:publisher.department":["Department of Molecular and Cell Biology"],"dc:subject":["Biochemistry"],"dc:title":["The primary structure of histone H2B from the mollusc Patella granatina"],"dc:type":["Doctoral Thesis"],"dc:type.qualificationlevel":["Doctoral","PhD"]},"updated_at":"2026-07-22T22:23:09Z"}