Expression, purification and characterisation of protoporphyrinogen oxidases from diverse species

This work involved the characterisation of protoporphyrinogen oxidase (PPO), the penultimate enzyme in haem biosynthesis, from Bacillus subtilis, Myxococcus xanthus, and human. A defect in human PPO causes variegate porphyria, an autosomal dominant disorder characterised by skin photosensitivity and propensity towards acute neurovisceral crises. At the beginning of this project little information was available on the kinetic and biophysical properties of isolated PPOs due largely to difficulties associated with their purification from natural sources.

Grantor dc:publisher.institution

Department of Medicine

Year dc:date.issued

2000

Author dc:creator

• Siziba, Kwanele Bennett

Advisors dc:contributor.advisor

• Meissner, Peter
• Corrigall, Anne

Language dc:language.iso

eng

Handle dc:identifier.uri

http://hdl.handle.net/11427/3464

OAI identifier oai:identifier

oai:open.uct.ac.za:11427/3464