Membrane shedding in kidney (MDCK) cells as revealed by covalent markers during quantification of endocytosis and transcytosis

Membrane traffic in polarised cells was investigated by growing Madin-Darby canine kidney (MOCK) cells on ·permeable polycarbonate filter supports which allowed access to both sides of the cell monolayer. Membrane glycoconjugates on the apical and basolateral cell surfaces were labelled enzymatically with ³H- and ¹⁴C-galactose, respectively, to provide covalent membrane markers. Experiments were done to quantitate membrane traffic during endocytosis at the respective plasma membrane domains and that due to transcytosis. Internalized label was quantitatively distinguished from label on the respective cell surface by its resistance to removal by glycosidases.

Grantor dc:publisher.institution

Division of Medical Biochemistry and Structural Biology

Year dc:date.issued

1991

Author dc:creator

• Godenir, Nicole

Advisor dc:contributor.advisor

• Thilo, Lutz

Language dc:language.iso

eng

Handle dc:identifier.uri

http://hdl.handle.net/11427/27141

OAI identifier oai:identifier

oai:open.uct.ac.za:11427/27141