Global ETD Search
Search theses and dissertations gathered from participating repositories worldwide. Every result links back to the library that holds it. No account is needed.
Results
Showing 1 to 20 of 54 for “"MDCK"”.
-
Endozytose des Ca2+-sensitiven hIK1-Kanals in migrierenden MDCK-F-Zellen
… des hIK1-HA-34-Plasmids in migrierende MDCK-F-Zellen war aufgrund der extrazellulären Lage des HA-34-Epitops im Kanalprotein eine Markierung der Kanäle mit Antikörpern in lebenden Zellen möglich. Die Immunfluoreszenzmikroskopie zeigte nach 37°C-in vivo-Inkubation der Zellen mit …
-
Role of matriptase in the regulation of epithelial barrier permeability studied using MDCK cells
… two!variants!of!MadinNDarby!canine!kidney!(MDCK)! cells!were! used,! together!with! the! “calciumNswitch”!model! of! epithelial! function! with!measurements!of!transepithelial!electrical!resistance!(TEER).! Inhibitors!of!matriptase!proteolytic!activity!delayed!the!restoration!of!TEER!after! …
-
Insulin-Like Growth Factor Binding Protein-6: Posttranslational modifications and sorting in polarized MDCK cells
… in der polarisierten Madin-Darby canine kidney (MDCK) Zelllinie. Die Daten ergaben, dass MDCK-Zellen verschiedene neutrale Proteasen sezernieren, die IGFBP-2 bis -5 in Fragmente definierter Größe spalten. IGFBP-6, das wichtigste sequestrierende Bindungsprotein für IGF II, wird unter transienter …
-
Membrane shedding in kidney (MDCK) cells as revealed by covalent markers during quantification of endocytosis and transcytosis
Membrane traffic in polarised cells was investigated by growing Madin-Darby canine kidney (MOCK) cells on ·permeable polycarbonate filter supports which allowed access to both sides of the cell monolayer. Membrane glycoconjugates on the apical and basolateral cell surfaces were labelled …
-
Transfektion migrierender Nierenepithelzellen mit dem Kalium-Kanal ROMK2
… Regionen von migrierenden Nierenepithelzellen (MDCK-F-Zellen) zu fin- den sind (z. B.: NHE1, Cl/HCO -Austauscher AE2). Diese reichern sich am Vor- derende der MDCK-F-Zellen an. Es war nicht bekannt, wo sich Markerproteine der apikalen Membran wiederfinden. Um dieser Frage nachzugehen, …
-
Activation of Ca<sup>2+</sup>-activated K<sup>+</sup> Channels and Cell Migration by Hepatocyte Growth Factor/Scatter Factor in Madin-Darby Canine Kidney Cells.
… a role in malignancy. Migration of transformed MDCK-F cells depends on activation of a charybdotoxin (ChTX)-sensitive, volume-activated membrane K<sup>+</sup> current. Patch-clamp electrophysiology and transwell migration assays were used to study the effects of HGF/SF on membrane K<sup>+</sup> …
-
Human Cytochrome P450 3A4 Over-Expressing IEC-18 and MDCK Cell Lines as an In-Vitro Model to Assess Gut Permeability and the Enzyme Metabolism
… gut and the liver. Mammalian cells lines such as MDCK and Caco-2 are commonly employed to assess drug permeability but they lack or have low expression level of drug metabolism enzyme expression such as CYP3A4, which contributes to significant first-pass gut and liver metabolism for many drugs. …
-
Purification of Vitamin D Binding Protein and Its Role in the Uptake of 25-Hydroxyvitamin D by Cultured Kidney Cells (Mdck Cells, Tissue Culture, Dbp)
This thesis reports the isolation of vitamin D binding protein (DBP) and its use in studies investigating the uptake of 25(OH)D(,3) by kidney cell lines. DBP was isolated from pig plasma in three steps: chromatography on DEAE cellulose and DEAE Sephadex, followed by chromatofocusing. The procedure …
-
Localization of Human Prostaglandin E2 Receptors in Polarized Epithelial Cells
… cells such as Madin-Darby canine kidney (MDCK) cells. Polarized epithelial cells are composed of apical and basolateral plasma membrane domains with specific protein compositions separated by tight junctions. Purinergic, muscarinic, and adrenergic receptors are a few examples of GPCRs that …
-
The biomechanical properties of epithelial cells and tissue in two and three dimensions
… this work, Madine-Darby Canine Kidney (MDCK) cells and cysts were used as a model for epithelial tissue. These cells can be genetically modified to mimic the onset of colorectal cancer, by the expression of a truncated Adenomatous polyposis coli (APC) protein: N-APC. N-APC is present in …
-
Nicotin beeinflußt die durch Influenza-A-Virus induzierte Apoptose in humanen und caninen Zellen
… und einer immortalisierten Hundenierenzellinie (MDCK) ergaben: <br> <br>1. Die durch Influenza-A-Virus-Infektion von primären humanen Monozyten und MDCK-Zellen ausgelöste Apoptose war mit Ausnahmen durch Nicotin hemmbar. <br>2. Die Inhibition der Apoptose durch Nicotin erhöhte in …
-
Organization and formation of the apical membrane of epithelial cells
… surface of untreated and raft lipid depleted MDCK cells with atomic force microscopy revealed that raft lipids are involved in the formation and/or maintenance of microvilli, actin based protrusions of the apical plasma membrane, indicating a regulatory link between membrane domains and the …
-
Tyrosine Phosphorylation of Villin: Effects on Actin Dynamics, Cell Morphology and Cell Migration
… villin in doxycyline-regulated HeLa Tet-off and MDCK Tet-off cells enhanced cell migration. We further demonstrate that tyrosine phosphorylation of villin by c-src is required for villin-induced cell migration. Previously, we identified four tyrosine phosphorylation sites in the amino-terminal …
-
Polarised secretion of leukaemia inhibitory factor
… exogenously in Madin-Darby canine kidney (MDCK) cells under the control of a tetracycline response promoter that allowed a variety of LIF expression levels to be tested. Exogenous murine LIF was secreted predominantly from the apical (60%) membrane of MDCK cells irrespective of the signal …
-
Protein sorting to the apical membrane of epithelial cells
… from two cell lines, Madin-Darby canine kidney (MDCK) and Jurkat cells, was compared. It was shown that the detergents differed considerably in their ability to selectively solubilise membrane proteins and lipids. These results make it unlikely that different DRMs reflect the same underlying …
-
Novel In-Vitro Approaches to Investigate Membrane Partitioning and pH-Dependent Passive Permeation of Small Molecule Drugs
… concentration in drug-spiked cell suspensions (MDCK & rat hepatocyte) was pursued with a novel in vitro microdialysis approach followed by in silico investigations with mathematical modeling. Depending on the drug, the apparent pKa values in the surrogate membrane system at different pH levels …
-
Charakterisierung des mit der Alzheimer Krankheit assoziierten Genprodukts Presenilin 1 und Interaktion mit beta-Catenin
… überprüft. In in SH-SY5Y Zellen und MDCK Zellen zeigte PS1 vor allem eine Anfärbung im Bereich von ER und Golgi, während es in primären, hippokampalen Neuronen auch im Bereich von Synapsen angefärbt wurde. b-Catenin war vor allem an der Plasmamembran und schwächer auch im Zellkern und …
-
Characterization of Molecular Glycerophospholipids by Quadrupole Time-of-Flight Mass Spectrometry
… composition of PCs of total lipid extracts of MDCK cells and of human red blood cells (RBC) could accurately be charted. Significant presence of positional isomers was observed increasing the total number of individual PC species close to one hundred. Secondly, the molecular PC and SM species …
-
Multifunctional Liposomal Carriers Encapsulating Temozolomide, RG7388 and Elacridar for Targeted Glioblastoma Multiforme Therapy
… that the liposomes showed lower toxicity in MDCK cells, while the toxicity for U87 and GBM 108 cells for the liposomal systems was higher than that of the drug solutions. Higher uptake for the folate decorated liposomes was seen in the U87 and GBM108 cell lines. The permeability of the …
-
Quantitative Measurements of Force Distribution in Single and Multi Cellular Systems
… past twenty years. Madin-Darby Canine Kidney (MDCK) cells form functional cellular aggregates, termed cysts under special conditions in vitro. The conditions were adapted to induce cyst growth on force sensor arrays allowing precise force mapping during cyst development. These data were …
Page 1 of 3