Cloning, expression, purification and drug targeting of Plasmodium falciparum hypoxanthine guanine xanthine phosphoribosyltransferase (HGXPRT)

The research concerns sub-cloning the gene for HGXPRT from Plasmodium falciparum from a vector with a His-tag facility to one without, expression of the protein in E. coli, and purification. On an analytical scale (40 ml culture), a purification procedure was developed that involves extraction of contaminating proteins by anion exchange chromatography (HGXPRT does not bind under the conditions used), followed by Reactive Red 120 agarose affinity chromatography.

Grantor dc:publisher.institution

Division of Chemical Pathology

Year dc:date.issued

2005

Author dc:creator

• Mbewe, Boniface

Advisors dc:contributor.advisor

• Mcintosh, David B
• Chibale, Kelly

Language dc:language.iso

eng

Handle dc:identifier.uri

http://hdl.handle.net/11427/2696

OAI identifier oai:identifier

oai:open.uct.ac.za:11427/2696