{"id":{"repo_id":"cape-town","oai_identifier":"oai:open.uct.ac.za:11427/2696"},"canonical_url":"https://search.dev.ndltd.org/etd/cape-town/oai:open.uct.ac.za:11427/2696","repository":{"repo_id":"cape-town","name":"University of Cape Town","base_url":"https://open.uct.ac.za/oai/request"},"display":{"title":"Cloning, expression, purification and drug targeting of Plasmodium falciparum hypoxanthine guanine xanthine phosphoribosyltransferase (HGXPRT)","abstract":"The research concerns sub-cloning the gene for HGXPRT from Plasmodium falciparum from a vector with a His-tag facility to one without, expression of the protein in E. coli, and purification. 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On an analytical scale (40 ml culture), a purification procedure was developed that involves extraction of contaminating proteins by anion exchange chromatography (HGXPRT does not bind under the conditions used), followed by Reactive Red 120 agarose affinity chromatography."]},{"key":"dc:title","label":"Title","values":["Cloning, expression, purification and drug targeting of Plasmodium falciparum hypoxanthine guanine xanthine phosphoribosyltransferase (HGXPRT)"]}]}],"canonical_facts":{"dc:contributor.advisor":["Mcintosh, David B","Chibale, Kelly"],"dc:creator":["Mbewe, Boniface"],"dc:date.accessioned":["2014-07-28T08:14:03Z"],"dc:date.available":["2014-07-28T08:14:03Z"],"dc:date.issued":["2005"],"dc:description":["Includes bibliographical references."],"dc:description.abstract":["The research concerns sub-cloning the gene for HGXPRT from Plasmodium falciparum from a vector with a His-tag facility to one without, expression of the protein in E. coli, and purification. 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