Molecular genetic characterization of two solvent pathway dehydrogenases from Clostridium Acetobutylicum

Clostridium acetobutylicum P262 is an endospore-forming Gram-positive anaerobic bacterium, which has been used for the industrial production of acetone and butanol from carbohydrate substrates. This study forms part of a wider research effort into the genetics and molecular biology of C. acetobutylicum, which has as an ultimate goal the commercial improvement, and a fundamental understanding of the ABE fermentation. The aim of this study was to isolate and characterize genes involved in solventogenesis. The cloning, expression and characterization of the terminal solventogenic butanol dehydrogenase gene ( adhl), and the central pathway β-hydroxybutyryl-CoA dehydrogenase gene (hbd), which form part of a but operon are described.

Grantor dc:publisher.institution

Department of Molecular and Cell Biology

Year dc:date.issued

1989

Author dc:creator

• Youngleson, Jonathan Sinclair

Advisors dc:contributor.advisor

• Woods, David R
• Jones, David T

Language dc:language.iso

eng

Handle dc:identifier.uri

http://hdl.handle.net/11427/23245

OAI identifier oai:identifier

oai:open.uct.ac.za:11427/23245