{"id":{"repo_id":"cape-town","oai_identifier":"oai:open.uct.ac.za:11427/23245"},"canonical_url":"https://search.dev.ndltd.org/etd/cape-town/oai:open.uct.ac.za:11427/23245","repository":{"repo_id":"cape-town","name":"University of Cape Town","base_url":"https://open.uct.ac.za/oai/request"},"display":{"title":"Molecular genetic characterization of two solvent pathway dehydrogenases from Clostridium Acetobutylicum","abstract":"Clostridium acetobutylicum P262 is an endospore-forming Gram-positive anaerobic bacterium, which has been used for the industrial production of acetone and butanol from carbohydrate substrates. This study forms part of a wider research effort into the genetics and molecular biology of C. acetobutylicum, which has as an ultimate goal the commercial improvement, and a fundamental understanding of the ABE fermentation. The aim of this study was to isolate and characterize genes involved in solventogenesis. The cloning, expression and characterization of the terminal solventogenic butanol dehydrogenase gene ( adhl), and the central pathway β-hydroxybutyryl-CoA dehydrogenase gene (hbd), which form part of a but operon are described.","abstract_html":"Clostridium acetobutylicum P262 is an endospore-forming Gram-positive anaerobic bacterium, which has been used for the industrial production of acetone and butanol from carbohydrate substrates. This study forms part of a wider research effort into the genetics and molecular biology of C. acetobutylicum, which has as an ultimate goal the commercial improvement, and a fundamental understanding of the ABE fermentation. The aim of this study was to isolate and characterize genes involved in solventogenesis. The cloning, expression and characterization of the terminal solventogenic butanol dehydrogenase gene ( adhl), and the central pathway β-hydroxybutyryl-CoA dehydrogenase gene (hbd), which form part of a but operon are described.","abstract_has_math":false,"creators":["Youngleson, Jonathan Sinclair"],"institution":"Department of Molecular and Cell Biology","degree_name":null,"degree_level":null,"degree_discipline":null,"degree_department":null,"school":null,"contributors":[],"advisors":["Woods, David R","Jones, David T"],"committee_chairs":[],"committee_members":[],"year":1989,"date_issued":"1989","date_published":"1989","updated_at":"2026-07-22T22:23:47Z","subjects":[],"languages":["eng"],"rights":[],"rights_urls":[],"identifier_entries":[]},"links":{"outbound_url":"http://hdl.handle.net/11427/23245","outbound_label":"Handle","outbound_source":"dc:identifier.uri"},"metadata_groups":[{"id":"people","label":"People","entries":[{"key":"dc:contributor.advisor","label":"Advisor","values":["Woods, David R","Jones, David T"]},{"key":"dc:creator","label":"Author","values":["Youngleson, Jonathan Sinclair"]}]},{"id":"academic_context","label":"Academic Context","entries":[{"key":"dc:date.accessioned","label":"Dc Date Accessioned","values":["2017-01-26T07:34:12Z"]},{"key":"dc:date.available","label":"Dc Date Available","values":["2017-01-26T07:34:12Z"]},{"key":"dc:date.issued","label":"Date","values":["1989"]},{"key":"dc:publisher.department","label":"Dc Publisher Department","values":["Department of Molecular and Cell Biology"]},{"key":"dc:publisher.institution","label":"Dc Publisher Institution","values":["University of Cape Town"]},{"key":"dc:type","label":"Dc Type","values":["Doctoral Thesis"]},{"key":"dc:type.qualificationlevel","label":"Dc Type Qualificationlevel","values":["Doctoral"]},{"key":"dc:type.qualificationname","label":"Dc Type Qualificationname","values":["PhD"]}]},{"id":"language_rights","label":"Language and Rights","entries":[{"key":"dc:language.iso","label":"Language (ISO)","values":["eng"]}]},{"id":"identifiers","label":"Identifiers","entries":[{"key":"dc:identifier.uri","label":"Identifier URI","values":["http://hdl.handle.net/11427/23245"]}]},{"id":"additional","label":"Additional Metadata","entries":[{"key":"dc:description.abstract","label":"Abstract","values":["Clostridium acetobutylicum P262 is an endospore-forming Gram-positive anaerobic bacterium, which has been used for the industrial production of acetone and butanol from carbohydrate substrates. This study forms part of a wider research effort into the genetics and molecular biology of C. acetobutylicum, which has as an ultimate goal the commercial improvement, and a fundamental understanding of the ABE fermentation. The aim of this study was to isolate and characterize genes involved in solventogenesis. The cloning, expression and characterization of the terminal solventogenic butanol dehydrogenase gene ( adhl), and the central pathway β-hydroxybutyryl-CoA dehydrogenase gene (hbd), which form part of a but operon are described."]},{"key":"dc:title","label":"Title","values":["Molecular genetic characterization of two solvent pathway dehydrogenases from Clostridium Acetobutylicum"]}]}],"canonical_facts":{"dc:contributor.advisor":["Woods, David R","Jones, David T"],"dc:creator":["Youngleson, Jonathan Sinclair"],"dc:date.accessioned":["2017-01-26T07:34:12Z"],"dc:date.available":["2017-01-26T07:34:12Z"],"dc:date.issued":["1989"],"dc:description.abstract":["Clostridium acetobutylicum P262 is an endospore-forming Gram-positive anaerobic bacterium, which has been used for the industrial production of acetone and butanol from carbohydrate substrates. 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The cloning, expression and characterization of the terminal solventogenic butanol dehydrogenase gene ( adhl), and the central pathway β-hydroxybutyryl-CoA dehydrogenase gene (hbd), which form part of a but operon are described."],"dc:identifier.uri":["http://hdl.handle.net/11427/23245"],"dc:language.iso":["eng"],"dc:publisher.department":["Department of Molecular and Cell Biology"],"dc:publisher.institution":["University of Cape Town"],"dc:title":["Molecular genetic characterization of two solvent pathway dehydrogenases from Clostridium Acetobutylicum"],"dc:type":["Doctoral Thesis"],"dc:type.qualificationlevel":["Doctoral"],"dc:type.qualificationname":["PhD"]},"updated_at":"2026-07-22T22:23:47Z"}