Investigation of the antioxidant activity of phenolic compounds in cell culture systems

This study focuses on newly synthesized polyphenolic compounds which were expected to be antioxidants in vivo. Antioxidants are compounds which at low concentrations can prevent or reduce the deleterious effects caused by reactive oxygen species (ROS) induced by UVA exposure of the skin. This study focuses on developing methods required to measure the effects of these synthesized compounds in ultraviolet radiation-exposed cultured skin cells. Biocatalytic synthesis of polyphenolic compounds was carried out using isolated enzymes, tyrosinase from Agaricus biosporus, and laccase from Trametes pubescens to produce oxidized oligomeric products.

Grantor dc:publisher.institution

Centre for Bioprocess Engineering Research

Year dc:date.issued

2009

Author dc:creator

• Zwane, Eunice Nonhlanhla

Advisor dc:contributor.advisor

• Burton, Stephanie Gail

Language dc:language.iso

eng

Handle dc:identifier.uri

http://hdl.handle.net/11427/12126

OAI identifier oai:identifier

oai:open.uct.ac.za:11427/12126