L-arginine overproduction in Corynebacterium glutamicum ATCC 13032

Corynebacterium glutamicum is widely used for the commercial production of a variety of amino acids, including L-lysine, L-glutamate and L-threonine. With the exception of Larginine, the biosynthesis and regulation of most of these compounds in this bacterium are relatively well characterised in the literature. The research presented here focuses on improving our understanding of the regulation of L-arginine biosynthesis in C. glutamicum. This was performed with the ultimate goal of creating strains capable of producing L-arginine commercially. A novel gene replacement system was initially used for the directed mutation of the Larginine biosynthetic gene cluster in C. glutamicum ATCC 13032. This was met with limited success, however, and the pK19mobsacB vector was thus adopted for further mutagenesis of this region.

Grantor dc:publisher.institution

Department of Molecular and Cell Biology

Year dc:date.issued

2009

Author dc:creator

• Theron, Grant de V

Advisor dc:contributor.advisor

• Reid, Sharon J

Language dc:language.iso

eng

Handle dc:identifier.uri

http://hdl.handle.net/11427/12122

OAI identifier oai:identifier

oai:open.uct.ac.za:11427/12122