{"id":{"repo_id":"cape-town","oai_identifier":"oai:open.uct.ac.za:11427/12122"},"canonical_url":"https://search.dev.ndltd.org/etd/cape-town/oai:open.uct.ac.za:11427/12122","repository":{"repo_id":"cape-town","name":"University of Cape Town","base_url":"https://open.uct.ac.za/oai/request"},"display":{"title":"L-arginine overproduction in Corynebacterium glutamicum ATCC 13032","abstract":"Corynebacterium glutamicum is widely used for the commercial production of a variety of amino acids, including L-lysine, L-glutamate and L-threonine. With the exception of Larginine, the biosynthesis and regulation of most of these compounds in this bacterium are relatively well characterised in the literature. The research presented here focuses on improving our understanding of the regulation of L-arginine biosynthesis in C. glutamicum. This was performed with the ultimate goal of creating strains capable of producing L-arginine commercially. A novel gene replacement system was initially used for the directed mutation of the Larginine biosynthetic gene cluster in C. glutamicum ATCC 13032. This was met with limited success, however, and the pK19mobsacB vector was thus adopted for further mutagenesis of this region.","abstract_html":"Corynebacterium glutamicum is widely used for the commercial production of a variety of amino acids, including L-lysine, L-glutamate and L-threonine. With the exception of Larginine, the biosynthesis and regulation of most of these compounds in this bacterium are relatively well characterised in the literature. The research presented here focuses on improving our understanding of the regulation of L-arginine biosynthesis in C. glutamicum. This was performed with the ultimate goal of creating strains capable of producing L-arginine commercially. A novel gene replacement system was initially used for the directed mutation of the Larginine biosynthetic gene cluster in C. glutamicum ATCC 13032. This was met with limited success, however, and the pK19mobsacB vector was thus adopted for further mutagenesis of this region.","abstract_has_math":false,"creators":["Theron, Grant de V"],"institution":"Department of Molecular and Cell Biology","degree_name":null,"degree_level":null,"degree_discipline":null,"degree_department":null,"school":null,"contributors":[],"advisors":["Reid, Sharon J"],"committee_chairs":[],"committee_members":[],"year":2009,"date_issued":"2009","date_published":"2009","updated_at":"2026-07-22T22:22:42Z","subjects":[],"languages":["eng"],"rights":[],"rights_urls":[],"identifier_entries":[]},"links":{"outbound_url":"http://hdl.handle.net/11427/12122","outbound_label":"Handle","outbound_source":"dc:identifier.uri"},"metadata_groups":[{"id":"people","label":"People","entries":[{"key":"dc:contributor.advisor","label":"Advisor","values":["Reid, Sharon J"]},{"key":"dc:creator","label":"Author","values":["Theron, Grant de V"]}]},{"id":"academic_context","label":"Academic Context","entries":[{"key":"dc:date.accessioned","label":"Dc Date Accessioned","values":["2015-01-13T03:59:24Z"]},{"key":"dc:date.available","label":"Dc Date Available","values":["2015-01-13T03:59:24Z"]},{"key":"dc:date.issued","label":"Date","values":["2009"]},{"key":"dc:publisher.department","label":"Dc Publisher Department","values":["Department of Molecular and Cell Biology"]},{"key":"dc:publisher.institution","label":"Dc Publisher Institution","values":["University of Cape Town"]},{"key":"dc:type","label":"Dc Type","values":["Doctoral Thesis"]},{"key":"dc:type.qualificationlevel","label":"Dc Type Qualificationlevel","values":["Doctoral"]},{"key":"dc:type.qualificationname","label":"Dc Type Qualificationname","values":["PhD"]}]},{"id":"language_rights","label":"Language and Rights","entries":[{"key":"dc:language.iso","label":"Language (ISO)","values":["eng"]}]},{"id":"identifiers","label":"Identifiers","entries":[{"key":"dc:identifier.uri","label":"Identifier URI","values":["http://hdl.handle.net/11427/12122"]}]},{"id":"additional","label":"Additional Metadata","entries":[{"key":"dc:description","label":"Description","values":["Includes abstract.","Includes bibliographical references (p. 155-174)."]},{"key":"dc:description.abstract","label":"Abstract","values":["Corynebacterium glutamicum is widely used for the commercial production of a variety of amino acids, including L-lysine, L-glutamate and L-threonine. With the exception of Larginine, the biosynthesis and regulation of most of these compounds in this bacterium are relatively well characterised in the literature. The research presented here focuses on improving our understanding of the regulation of L-arginine biosynthesis in C. glutamicum. This was performed with the ultimate goal of creating strains capable of producing L-arginine commercially. A novel gene replacement system was initially used for the directed mutation of the Larginine biosynthetic gene cluster in C. glutamicum ATCC 13032. 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The research presented here focuses on improving our understanding of the regulation of L-arginine biosynthesis in C. glutamicum. This was performed with the ultimate goal of creating strains capable of producing L-arginine commercially. A novel gene replacement system was initially used for the directed mutation of the Larginine biosynthetic gene cluster in C. glutamicum ATCC 13032. This was met with limited success, however, and the pK19mobsacB vector was thus adopted for further mutagenesis of this region."],"dc:identifier.uri":["http://hdl.handle.net/11427/12122"],"dc:language.iso":["eng"],"dc:publisher.department":["Department of Molecular and Cell Biology"],"dc:publisher.institution":["University of Cape Town"],"dc:title":["L-arginine overproduction in Corynebacterium glutamicum ATCC 13032"],"dc:type":["Doctoral Thesis"],"dc:type.qualificationlevel":["Doctoral"],"dc:type.qualificationname":["PhD"]},"updated_at":"2026-07-22T22:22:42Z"}