Over-expression of NRF-1 in C2C12 myotubes increases GLUT4 content via a transcriptional cascade involving MEF2A

Previous studies have shown that over-expression of nuclear respiratory factor (NRF)-1 in mice increases glucose transporter (GLUT)-4 and myocyte enhancer factor (MEF-) 2A content, but the mechanisms have not been elucidated. Because NRF-1 has a binding site on the mef2a gene, and MEF2A binds the glut4 gene as a MEF2A-MEF2D heterodimer, the aims of this study were to determine whether NRF-1 over-expression a) enhanced GLUT4 expression indirectly via MEF2A and b) alters MEF2A-MEF2D dimer formation in C2C12 myotubes.

Grantor dc:publisher.institution

Department of Molecular and Cell Biology

Year dc:date.issued

2012

Author dc:creator

• Gumede, Dimakatso

Advisor dc:contributor.advisor

• Ojuka, Edward

Language dc:language.iso

eng

Handle dc:identifier.uri

http://hdl.handle.net/11427/11772

OAI identifier oai:identifier

oai:open.uct.ac.za:11427/11772