An integrative approach to studying protein-protein interactions within the genome of Mycobacterium tuberculosis

Proteins mediate bio-chemical processes in cellular organisms by functioning as structural components, signaling molecules, enzymes, transcription factors and receptors, through complex interactions that they make with one another. The study of protein-protein interactions (PPI) within an organism is thus a vital step towards understanding the cellular life process of an organism. PPIs have been studied by specially designed experiments to identify pairs of proteins that are suspected to interact. Experimental approaches are quite expensive, yet still there are cases of wrongly identified protein-protein interactions. In this study, we predict PPIs computationally by exploiting known properties of proteins and PPIs and the requirement that proteins be present in the same cell compartment at the same time in order to interact (subcellular localization and gene expression).

Grantor dc:publisher.institution

Department of Molecular and Cell Biology

Year dc:date.issued

2011

Author dc:creator

• Opap, Kenneth Babu

Advisor dc:contributor.advisor

• Mulder, Nicola

Language dc:language.iso

eng

Handle dc:identifier.uri

http://hdl.handle.net/11427/11086

OAI identifier oai:identifier

oai:open.uct.ac.za:11427/11086