Regulation of Oligodendrocyte differentiation through Neuroligin-Neurexin signalling

The neuroligin (NL) gene family has been well characterized in neurons by virtue of its role as a synaptic cell adhesion molecule, and because mutations in the gene have been associated with Autism Spectrum Disorder (ASD). However much less is known about its role in the glial cell population, where one of the subtypes, neuroligin 3, is expressed. The work presented here investigates NL’s role in oligodendrocytes (OLs), a subtype of glial cells responsible for the myelination in the central nervous system. This was accomplished by first determining NL’s presence in this cell type in hippocampal dissociated cultures by immunocytochemistry and in situ hybridization. It was found that by interfering with the interaction of NL and its pre-synaptic partner neurexin (NX), the developmental profile of OL cells was delayed. Conversely, enhancing the interaction accelerated the developmental profile. Evidence is also presented that glutamate receptors are potentially spatially localized to the contact points between oligodendrocytes and axons in response to the interaction between NL and NX. As mutations in NL have been linked to autism, this provides a new potential mechanism by which the cognitive deficits observed could occur.

Name thesis:degree_name

Master of Science (MSc)

Discipline thesis:degree_discipline

Neuroscience

Grantor dc:publisher.institution

Graduate Studies

Year dc:date.issued

2013

Author dc:creator

• Scott, Lucas O.M.

Advisor dc:contributor.advisor

• Colicos, Michael

• Neuroscience

Statement dc:rights

• University of Calgary graduate students retain copyright ownership and moral rights for their thesis. You may use this material in any way that is permitted by the Copyright Act or through licensing that has been assigned to the document. For uses that are not allowable under copyright legislation or licensing, you are required to seek permission.

Language dc:language.iso

eng

DOI

http://dx.doi.org/10.11575/PRISM/27587

OAI identifier oai:identifier

oai:ucalgary.scholaris.ca:11023/595