The Immune Modulatory Effects of Tylvalosin in Porcine Neutrophils and Macrophages in vitro

Tylavlosin (TYL), a veterinary macrolide antibiotic, has been reported to have superior efficacy treating bacterial infections of the respiratory tract in swine. This study aims to characterize potential pro-resolving of tylvalosin benefits in a porcine model by elucidating its effects, in isolated neutrophils and monocyte-derived macrophages from piglets. Our findings indicate that TYL increases porcine neutrophil and macrophage apoptosis in a dose-dependent and time-dependent manners, without affecting levels of necrosis. TYL also modulates mediators of inflammation, by increasing pro-resolving lipid mediators (LXA4 and RvD1) in neutrophils and down regulating the pro- inflammatory mediators in stimulated neutrophils (LTB4) and in stimulated macrophages (CXCL8 and IL-1α). Together, these findings demonstrate that tylvalosin has immuno- modulating properties in vitro, including the induction of leukocyte apoptosis, the inhibition of pro-inflammatory cytokines and lipids mediators, and the induction of pro- resolving lipid mediators. Future studies using live piglets will help determine whether and how these effects may translate into anti-inflammatory benefits in the content of inflammatory disease within the lung.

Name thesis:degree_name

Master of Science (MSc)

Discipline thesis:degree_discipline

Biological Sciences

Grantor dc:publisher.institution

Graduate Studies

Year dc:date.issued

2017

Author dc:creator

• Moges, Ruth

Advisor dc:contributor.advisor

• Buret, Andre

Committee members dc:contributor.committeemember

• Morck, Douglas
• Storey, Douglas

• Microbiology

Statement dc:rights

• University of Calgary graduate students retain copyright ownership and moral rights for their thesis. You may use this material in any way that is permitted by the Copyright Act or through licensing that has been assigned to the document. For uses that are not allowable under copyright legislation or licensing, you are required to seek permission.

Language dc:language.iso

eng

DOI

http://dx.doi.org/10.11575/PRISM/28175

OAI identifier oai:identifier

oai:ucalgary.scholaris.ca:11023/3941