Characteristics of Donor-Specific anti-HLA Antibodies (DSA) Impacting different Renal Allograft Outcomes

AMR is the major complication of renal transplantation that significantly affects the allograft survival. Complement fixation of Donor specific anti-HLA antibodies is the hallmark of AMR. Different methods have been developed to detect harmful DSAs. However, not all DSAs have the same detrimental effect, as different antibody characteristics (timing, specificity, strength, and complement-fixing ability) might trigger distinct immune responses. Here, we assessed DSA characteristics in renal transplant patients. Thirty-nine patients with de novo DSA were analyzed for the DSA MFI values, C1q positivity and IgG subclasses. IgG1 was the predominant IgG subclass (49.4%), followed by IgG3 (24.7%), IgG2 (16%), and IgG4 (9.9%). Among DSA characteristics, DSA MFI and IgG1 subclass were strongly correlated with C1q positivity (p= 0.01 and p= 0.009, respectively). Further studies are needed to investigate the clinical relevance of high MFI DSA and IgG1 subclass in improving the utility of the C1q assay in predicting graft outcomes.

Name thesis:degree_name

Master of Science (MSc)

Discipline thesis:degree_discipline

Medical Science

Grantor dc:publisher.institution

Graduate Studies

Year dc:date.issued

2015

Author dc:creator

• Ghandorah, Salim

Advisors dc:contributor.advisor

• Berka, Noureddine
• Khan, Faisal

• Immunology

Statement dc:rights

• University of Calgary graduate students retain copyright ownership and moral rights for their thesis. You may use this material in any way that is permitted by the Copyright Act or through licensing that has been assigned to the document. For uses that are not allowable under copyright legislation or licensing, you are required to seek permission.

Language dc:language.iso

eng

DOI

http://dx.doi.org/10.11575/PRISM/25256

OAI identifier oai:identifier

oai:ucalgary.scholaris.ca:11023/2148