Sources of Variability in a Proteomic Experiment

The study of proteomics holds the hope for detecting serious diseases earlier than is currently possible by analyzing blood samples in a mass spectrometer. Unfortunately, the statistics involved in comparing a control group to a diseased group are not trivial, and these difficulties have led others to incorrect decisions in the past. This paper considers a nested design that was used to quantify and identify the sources of variation in the mass spectrometer at BYU, so that correct conclusions can be drawn from blood samples analyzed in proteomics. Algorithms were developed which detect, align, correct, and cluster the peaks in this experiment. The variation in the m/z values as well as the variation in the intensities was studied, and the nested nature of the design allowed us to estimate the sources of that variation. The variation due to the machine components, including the mass spectrometer itself, was much greater than the variation in the preprocessing steps. This conclusion inspires future studies to investigate which part of the machine steps is causing the most variation.

Name thesis:degree_name

MS

Grantor dc:publisher

Brigham Young University - Provo

Author dc:creator

• Crawford, Scott Daniel

• proteomics
• variance components
• mass spectrometer
• Statistics and Probability

Language dc:language

English

Repository record dc:identifier

https://scholarsarchive.byu.edu/etd/776

OAI identifier oai:identifier

oai:scholarsarchive.byu.edu:etd-1775