OVERCOMING THE LIMITATIONS OF TISSUE ENGINEERED SCAFFOLDS FOR DENTAL ROOT REPAIR

Major requirements of tissue-engineered scaffolds for any purpose are to demonstrate their initial sterility, and to demonstrate their ability to exchange nutrients/wastes while receiving sufficient interior oxygenation. In the special case of cell-growth scaffolds to be used for endodontic purposes, the biomaterial constructs must also be long and thin while remaining sufficiently porous and strong to allow cellular growth within often irregular and narrow root canals. This research evaluated the prospects that crafted narrow scaffolds of sintered, biodegradable Bioglass could initiate and sustain osteoblastic or odontoblastic ingrowth via interior channels introduced by sacrificial filaments of fibers and/or perforated tubing. The work confirmed that the high temperatures of the sintering process sufficiently sterilized the scaffolds to allow their immediate clinical use from sterile containers. The presence of an interconnectivity channel system via burn out of porous polypropylene oxygenator membranes allows for proper nutrient delivery to the infiltrating cells. Blackened carbon-coated scaffolds acted as a filter for the media, resulting in no nutrient delivery to the cells. The new white scaffolds did not filter the media but their high alkaline pH caused necrosis of all cells. Future work should be done to neutralize the pH by finding an appropriate volume to sample ratio to be able to overcome the initial pH flush.

Grantor dc:publisher

State University of New York at Buffalo

Year dc:date.issued

2018

Author dc:creator

• McCune, Jenna

Contributors dc:contributor

• Baier, Robert
• Biomaterials

• materials science

Statement dc:rights

• Users of works found in University at Buffalo Institutional Repository (UBIR) are responsible for identifying and contacting the copyright owner for permission to reuse. University at Buffalo Libraries do not manage rights for copyright-protected works and cannot assist with permissions.
• Copyright retained by author.

Language dc:language

eng

Handle dc:identifier

http://hdl.handle.net/10477/78071

OAI record oai:identifier

https://ubir.buffalo.edu/oai/request?verb=GetRecord&metadataPrefix=qdc&identifier=oai%3Aubir.buffalo.edu%3A10477%2F78071