Computational Methods for Haplotype-aware De Novo Genome Assembly from Long Reads

Genomes of the same species usually show high diversity due to genetic variations. Most eukaryotes involve multiple copies of genomes, each of which is inherited from one of the ancestors. Whereas prokaryotes, such as virus and bacteria, are prone to form a collection of closely related strains which differ by small amounts of variants in the infected hosts or environment. Determining the DNA sequence of each genome copy and strain (together are called haplotype here), which is referred to as haplotype-aware genome assembly, plays a crucial role in genomics, medicine, and many other disciplines, because biological functionalities or phenotypic appearance can differ substantially across different haplotypes. Over the last few years, long-read sequencing technologies such as Pacific Biosciences and Oxford Nanopore Technologies, have greatly improved haplotype phasing and genome assembly because of the tremendous advantages in terms of read length. Nevertheless, the vast majority of existing long-read haplotype phasing or genome assembly tools rely on a pre-known high-quality reference as a backbone, or collapse homologous sequences into one consensus sequence, respectively, which introduces disturbing biases or fails to capture the haplotype diversity of heterogeneous genomes. <br /><br /> In this dissertation, several novel computational approaches are proposed to haplotype-aware de novo genome assembly by only using long-read sequencing data, which are particularly applied in diploid genome, viral quasispecies, polyploid genome and metagenome, respectively. Briefly, these methods firstly perform haplotype-aware sequencing error correction using different strategies, and subsequently construct haplotype-aware sequence graphs to generate haplotype-specific assemblies, which together enable to avoid reference biases on one hand and preserve haplotype diversity on the other. Benchmarking experiments on simulated and real datasets of varying complexity and diversity demonstrate that these methods drastically outperforms other state-of-the-art tools in terms of various evaluation criteria related to genome assembly, especially haplotype completeness and accuracy. In addition, these approaches presented in the thesis have been implemented as easy-to-use open-source tools.

Level thesis:degree_level

thesis.doctoral

Grantor dc:publisher

Universität Bielefeld

Year

2023

Author dc:creator

• Luo, Xiao

Repository record source_url

https://pub.uni-bielefeld.de/record/2969798

OAI identifier oai:identifier

oai:pub.uni-bielefeld.de:2969798