Studies into the release mechanisms of human placental lactogen in vitro

A procedure to study the in vitro release of HPL was developed. Hormone release, partially uncoupled from hormone biosynthesis, was measured by incubating placental fragments in physiological salt solutions for short periods. The effect of test substances and different ionic environments on HPL release were tested to ascertain the roles of calcium ion and cyclic AMP in the release mechanism. 2. The HPL concentration of the incubation media was measured with a specific radioimmunoassay. Particular features of the assay were the use of high concentrations of reagents, and the separation of antibody-bound and free hormone by ethanol fractionation. The assay had a useful range of 75-800 ng HPL/m1 with interassay and intra-assay variations of 14.7% and 3.2%, respectively.

Name thesis:degree_name

PhD

Level thesis:degree_level

Doctoral

Discipline thesis:degree_discipline

Obstetrics and Gynaecology

Grantor dc:publisher

ResearchSpace@Auckland

Year dc:date.issued

1977

Author dc:creator

• Choy, Vernon John

Advisor dc:contributor.advisor

• Dr Wayne B. Watkins

Statement dc:rights

• Items in ResearchSpace are protected by copyright, with all rights reserved, unless otherwise indicated.

Licence dc:rights.uri

• https://researchspace.auckland.ac.nz/docs/uoa-docs/rights.htm

Language dc:language.iso

en

Handle dc:identifier.uri

https://hdl.handle.net/2292/3113

OAI identifier oai:identifier

oai:researchspace.auckland.ac.nz:2292/3113