{"id":{"repo_id":"auckland-ms","oai_identifier":"oai:researchspace.auckland.ac.nz:2292/3113"},"canonical_url":"https://search.dev.ndltd.org/etd/auckland-ms/oai:researchspace.auckland.ac.nz:2292/3113","repository":{"repo_id":"auckland-ms","name":"University of Auckland","base_url":"https://researchspace.auckland.ac.nz/server/oai/request"},"display":{"title":"Studies into the release mechanisms of human placental lactogen in vitro","abstract":"A procedure to study the in vitro release of HPL was developed. Hormone release, partially uncoupled from hormone biosynthesis, was measured by incubating placental fragments in physiological salt solutions for short periods. The effect of test substances and different ionic environments on HPL release were tested to ascertain the roles of calcium ion and cyclic AMP in the release mechanism. 2. The HPL concentration of the incubation media was measured with a specific radioimmunoassay. Particular features of the assay were the use of high concentrations of reagents, and the separation of antibody-bound and free hormone by ethanol fractionation. The assay had a useful range of 75-800 ng HPL/m1 with interassay and intra-assay variations of 14.7% and 3.2%, respectively.","abstract_html":"A procedure to study the in vitro release of HPL was developed. Hormone release, partially uncoupled from hormone biosynthesis, was measured by incubating placental fragments in physiological salt solutions for short periods. The effect of test substances and different ionic environments on HPL release were tested to ascertain the roles of calcium ion and cyclic AMP in the release mechanism. 2. The HPL concentration of the incubation media was measured with a specific radioimmunoassay. Particular features of the assay were the use of high concentrations of reagents, and the separation of antibody-bound and free hormone by ethanol fractionation. 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