Extraction and Purification of Gamma-Tocotrienol from Rice Bran Oil Deodorizer Distillate

<p>The tocols (alpha-, beta-, gamma- and delta- tocopherols (T); alpha-, beta-, gamma- and delta-tocotrienols (T3)) make up the vitamin E family of antioxidants. Tocols have many health benefits. Gamma-T3 has been shown to protect against harmful radiation effects in mice. During rice bran oil refining up to 90% of tocols in rice bran are lost to the deodorizer distillate. The objective of this study was to optimize parameters for analyzing and isolating tocols from rice bran oil deodorizer distillate (RBODD). NP-HPLC analysis of tocol standards was optimized by testing ten mobile phases. Tocol extraction was optimized by testing three concentration solvents (acetonitrile, methanol, ethanol) using various solvent:RBODD ratios (5:1, 10:1, 15:1, 20:1) at two temperatures (4°C, -20°C). Hexane (HX)-ethyl acetate (EA)-acetic acid (AA) (97.3:1.8:0.9 v/v/v) provided baseline resolution of all eight tocols. All interactions including solvent*ratio*temperature had a significant effect on tocol levels in the extracts (p</p>

Name thesis:degree_name

Master of Science in Food Science (MS)

Level thesis:degree_level

Thesis

Year dc:date.available

2013

Author dc:creator

• Kordsmeier, Mary

Advisor dc:contributor.advisor

• Howard, Luke R.

Contributors dc:contributor

• Proctor, Andrew
• Allison, Neil T.

• Biological sciences
• Extraction
• Flash chromatography
• Food science
• Hplc analysis
• Tocotrienol
• Vitamin e
• Food Chemistry
• Food Processing

Repository record dc:identifier

https://scholarworks.uark.edu/etd/705

OAI identifier oai:identifier

oai:scholarworks.uark.edu:etd-1704