Upstream conserved sequence element that regulates TIM-3 induction in CD4+ T cell

The T cell immunoglobulin domain and mucin domain-3 (TIM-3) was discovered in Th1 and has been known as a T cell regulatory molecule. TIM-3 expression is induced by T cell activation through mechanism not yet well-investigated. In the study, I wanted to find DNA region involved in regulation of TIM-3 transcription in human CD4+ T cells between -70 kbp to -1 bp from the TIM-3 transcription start site. Given that regulatory DNA regions show nucleotide sequence homology between species, four conserved non-coding sequences (CNS -29, CNS -53, CNS -56 and CNS -63) were analyzed by comparison human TIM-3 upstream DNA sequences with mouse and cow counterparts. Only at CNS -63, H3K4 dimethylation that is associated with transcriptional activation was observed in Jurkat T cells and CD4+ T cells isolated from human blood. Further, CNS -63 increased luciferase reporter gene expression downstream of TIM-3 minimal promoter. Enhancer activity of CNS -63 was abrogated by mutation of putative NF-κB binding sites between -63423 and -63433. These results indicate that TIM-3 CNS -63 may regulate TIM-3 transcription in human CD4+ T cells.

Author dc:creator

• Komori, Kuniharu

Contributors dc:contributor

• 박, 선
• 대학원 의생명과학과
• 201124340

• TIM-3
• Histone H3 methylatio
• Transcriptionregulatio

Language dc:language

ko

Identifier

http://dcoll.ajou.ac.kr:9080/dcollection/jsp/common/DcLoOrgPer.jsp?sItemId=000000013572
000000013572

OAI identifier oai:identifier

oai:repository.ajou.ac.kr:201003/8590