Radiologic, Histologic and Functional Evaluation after Implantation of Cultured Trachea Allograft : in vivo study

BACKGROUND: Commonly known as praying about how hard of a reconstruction, cartilage grafts, anastomosis country EP and the glass plate or Sophie. The sum of the areas of narrowing it after removing the door if possible, all the way to the ideal treatment in patients with anastomosis hard liquor, this is not applicable. The sum of the door hard impossible to treat successfully a narrowing airway occlusion and remove enough parts that parts of the Agency's shortcomings, which are caused by you and will need to refinance. Shortcomings in order to rebuild parts of the EP-let, such as alcohol or commonly cartilage tissue is used, this is limited, the new agency's buildings and are willing to use water injection attempt. But in this case, rebuilding and injection duration, short after the additional aid is necessary, and this plant was destroyed or inflammation of the immune response and can lead to complications. The Agency, in the case of the plants, as well as the reconstruction and rebirth of cartilage parts played quickly and the mucous membrane and want to make the most important. The new agency's buildings or infusion water has persisted. OBJECT: In this study, the natural material pig cartilage extracellular using synthetic materials and made with natural ingredients to overcome the shortcomings of delay and maximize the benefits to increase the mechanical strength of the perforated scales and implanted in the rabbit with the support agencies, the Agency seeks to make research the possibility of reconstruction. MATERIALS AND METHODS: Pig cartilage cells and crushing ride throughout the process and after compression, produced in powder form as a liaison, salt extraction by third dimension PCS (Porcine Cartilage Substance derived Scaffold) produced 5 mm in height, diameter and 12mm. Allied to the scaffolds created from the knee cartilage cells of the Hare (1x106cells/scaffold) is implanted by inoculated for the strength and characteristics of the organization intends to give 7 weekly culture. 4 of the scaffolds that cultivate adult rabbit mm x 6 mm (diameter x height) institutional shortcomings were implants, 2 weeks, 1 month, 2 months later the human enemy, histological, video Byrne, features the forensic observation examinations. RESULTS: The physics of PCS support characteristics produced by observation, pore size is 250 ~ 350um was determined to be, was confirmed as the pole rate is 82% of the ball. Live/Dead viability assay result of enforcing support inside the cells could survive and more than 90%. Support I've checked with the naked eye, 1 week, 2 weeks, and keep the original shape of the 4 weeks the viscosity of the material and could see a slippery character. Transplant two weeks after endoscopic observations on the shortcomings of the transplant support or variation were observed. One month after transplant, the part of the authorities towards the inside of the parenting support organization could determine to form. Transplant after two months, one month has not been identified, was found in the parenting organizations, agencies are playing the parts of the internal membranes of appropriation and clarified that the boundaries of the scaffolds were observed. If you look at the results from the dye H&E 2 weeks after transplant, transplant initial transplanted cartilage cells by extracellular temperament is created and, in the medium term the surrounding extracellular attachment of macrophages and inflammation response by parenting organizations are formed, mature stage as the generated form with extracellular temperament is attributed to the phenomenon, drop-off child care organization would. As a result of the conduct dye Safranin-O the passage of time after transplantation to produce extracellular could see that. Cilia motility measurement result normal cilia and transplanted Cilia move, you will see a big difference with an invisible and, over time, the normal cilia and similarly could see that recovery. CONCLUSION: In vivo experiments obtained from a rabbit through the cultivation of the cells and establish how the cartilage in-class organizations improving and building institutions of Temperance in the experimental animal model about technology and clinical research and basic materials needed to apply to be a judge.

Author dc:creator

• 이, 범희

Contributors dc:contributor

• 김, 철호
• 대학원 의생명과학과
• 201124157

• 기관
• 조직공학
• 기관재생
• 연골세포
• 상피세포 섬모운동
• 세포 외 기질
• 돼지연골세포 지지체
• trachea
• tissue engineering
• tracheal regeneration
• chondrocyte
• Ciliary beat frequency
• extracllular matrix
• Porcine cartilage derived substance scaffold

Language dc:language

ko

Identifier

http://dcoll.ajou.ac.kr:9080/dcollection/jsp/common/DcLoOrgPer.jsp?sItemId=000000013543
000000013543

OAI identifier oai:identifier

oai:repository.ajou.ac.kr:201003/8589