Role of ICAM-1, a cell adhesion molecule, in morphological changes of BV2 microglial cell

Microglial cells, the major immune effector cells in the central nervous system, are activated in various brain injuries. Lipopolysaccharide (LPS), found in the outer membrane of Gram-negative bacteria, one of the major activator of microglia induces morphological changes and activation of the microglial cells. There are a series of cell adhesion molecules (CAM) related with these changes. In our present study, we studied the role of adhesion molecules including Intracellular adhesion molecule-1 (ICAM-1), which are responsible for the microglial activation using immortalized BV2 microglial cell line. After seeding in the plastic, BV2 cells showed their morphological changes, from the round shape to bipolar or multipolar (spread) shape, according to time course. The treatment of LPS (1 ㎍/ml) influenced their morphological change at earlier time, no longer than 6 hrs. LPS treatment increased the numbers of cells into spread form with short and large proximal processes and their distance ramification reduced, which were coincided with the mRNA and protein expression of ICAM-1. The monoclonal neutralizing antibody to ICAM-1 significantly reduced the numbers of the multipolar cells. However, there was no change in other cell adhesion molecules like LFA-1, MAC-1 and β1 integrin a subunit of fibronectin receptor α5β1 even in LPS treated group. LPS also increase the trace number of ramified cells. Therefore; ICAM-1 might play an important role for the LPS-induced morphological changes.

Author dc:creator

• Barua, Sumit

Contributors dc:contributor

• 백, 은주
• 대학원 의생명과학과
• 200924346

• BV2 미세아교세포
• 세포부착물질
• 면역세포
• ICAM-1
• BV2 microglial cell

Language dc:language

en

Identifier

http://dcoll.ajou.ac.kr:9080/dcollection/jsp/common/DcLoOrgPer.jsp?sItemId=000000011413
000000011413

OAI identifier oai:identifier

oai:repository.ajou.ac.kr:201003/4350