Cytopathic Effects of a Pathogenic Naegleria fowleri and an Anti-Nfa1 Antibody on Rat Microglial Cells

Naegleria fowleri, a free-living amoeba, causes fatal primary amoebic meningoencephalitis (PAME) in experimental animals and humans. On the pathogenic mechanisms of N. fowleri concerned with host tissue invasion, the adherence of amoeba to host cells is most important. The nfa1 gene (360 bp) was previously cloned from a cDNA library of pathogenic N. fowleri by immunoscreening, and produced a 13.1 kDa recombinant protein (rNfa1) that showed the pseudopodia specific localization by immunocytochemistry. On the basis of an idea that the pseudopodia-specific Nfa1 protein seems to be involved in the pathogenicity of N. fowleri, the cytopathic activity of N. fowleri trophozoites co-cultured with rat microglial cells was observed, and effects of an anti­Nfa1 antibody as treating on a co-culture system was elucidated. By a light, scanning and transmission electron microscope, N. fowleri trophozoites contacted with microglial cells produced vigorous pseudopodia and food­cups structure. Microglial cells were destroyed by N. fowleri trophozoites as showing the necrotic and apoptotic cell death in a time­dependent manner. As the results of ^(51)Cr release assay, N. fowleri showed 17.8, 24.9, 54.6 and 98.0% cytotoxicity against microglial cells at 3, 6, 12 and 24 hr post­incubation, respectively. On contrary, N. fowleri treated with an anti­Nfa1 antibody (1:100 dilution) showed 15.5, 20.3, 50.7 and 66.9% cytotoxicity, respectively. And then, microglial cells co-cultured with N. fowleri trophozoites secreted cytokines, TNF-α, IL-1β and IL-6, as the protective immune response.

Author dc:creator

• 오, 영환

Contributors dc:contributor

• 신, 호준
• 대학원 의학과
• 200324274

• Naegleria fowleri
• PAME
• nfa1 gene
• microglial cell
• anti-Nfa1 antibody
• cytotoxicity
• 파울러자유아메바
• 원발성 아메바성 수막뇌염
• nfa1 유전자
• 항 Nfa1 항체
• 세포독성

Language dc:language

en

Identifier

http://dcoll.ajou.ac.kr:9080/dcollection/jsp/common/DcLoOrgPer.jsp?sItemId=000000000088
000000000088

OAI identifier oai:identifier

oai:repository.ajou.ac.kr:201003/2286