Gonadotropin-Releasing Hormone Agonist Treatments Normalize Aromatase and Reduce Cyclooxygenase-2 in Ovarian Endometrioma and Eutopic Endometrium with Endometrosis

PURPOSE: To investigate whether GnRH agonists could normalize aromatase cytochrome P450 and cyclooxygenase-2 metabolism by direct action in the eutopic endometrium of endometriosis and ovarian endometrioma. METHODS: Endometrial specimens and ovarian endometrioma tissues were obtained from infertility women undergoing laparoscopic surgery. Biopsy samples of the endometrium were obtained before and after GnRH agonist therapy. The stromal cells of eutopic endometrium with endometroisis and ovarian endometrioma were cultured in the presence of GnRH agonist(leuprolide acetate 0, 1, 5, and 10 uM) for 24 hours. The expression of aromatase cytochrome P450 and COX-2 was examed by Western blot. RESULTS: Protein expression of aromatase cytochrome P450 and COX-2 was reduced in the eutopic endometrium of patients treated with GnRH agonist for 3 months. The stromal cells culture of endometrial explants and ovarian endometrioma by GnRH agonist reduced aromatase cytochrome P450 and COX-2. Phosphorylated ERK was also reduced in endometriotic stromal cultures by GnRH agonist. CONCLUSION: Therapy with GnRH agonists decreased expression of aromatase cytochrome P450 and COX-2 in eutopic endometrium of endometriosis and ovarian endometrioma. GnRH agonist reduced the expression of aromatase cytochrome P450 and COX-2 by promoting a hypoestrogenic state and direct action on eutopic endometrium and ovarian endometrioma.

Author dc:creator

• 김, 영아

Contributors dc:contributor

• 황, 경주
• 대학원 의학과
• 200424303

• 자궁내막증
• 성선자극호르몬 분비호르몬 효능제
• 아로마타제
• COX-2
• 기질세포배양

Language dc:language

ko

Identifier

http://dcoll.ajou.ac.kr:9080/dcollection/jsp/common/DcLoOrgPer.jsp?sItemId=000000002094
000000002094

OAI identifier oai:identifier

oai:repository.ajou.ac.kr:201003/1649