Neuronal Differentiation and Functional Recovery after Transplantation of Human Mesenchymal Stem Cell in The Ischemic Rat Model

Brain transplantation had offered as an effective treatment for patients suffering from neurodegenerative diseases, including Parkinson's disease, Huntington's disease and Stroke. Human mesenchymal stem cells are pluripotent stem cells, which can be differentiated into mesenchymal lineage cells. Those cells from the individual patient have the advantage to minimize the immune rejection. In this study, cytokine-inducted human mesenchymal stem cells were transplanted via tail vein into cerebral ischemic rat model exposed to transient MCA occlusion. We examined that cytokine-inducted human mesenchymal stem cells transplanted in brain had survived and differentiated into neural cells and neurological functions were improved after stroke in the rats. Adult female Sprague-Dawley rats were anesthetized with chloral hydrate, and ischemic lesions were made in the rats by transient MCA occlusion lasted for 120 minutes. One day later, cytokine-inducted human mesenchymal stem cells carrying LacZ gene were implanted via tail vein. The rats were assessed for sensory-motor function and sacrificed for histologic examination at 7, 14, 28, 56 days after transplantation. Immunohistochemical staining was used to identify cells derived from human mesenchymal stem cells. A large number of X-galactosidase positive human mesenchymal stem cells were observed in the ischemic core and ischemic boundary zone in the rat brain. Some human mesenchymal stem cells were reactive for the astrocytic marker - glial fibrillary acidic protein(GFAP) and neuronal marker - neuronal nuclear antigen(NeuN). These findings show that some cytokine-inducted human mesenchymal stem cells were differentiated into neurons and astrocytes to replace the damaged neural cells in the rats of cerebral stroke. The rats that were transplanted with cytokine-inducted human mesenchymal stem cells showed slightly better functional improvement than control groups and the rats with human mesenchymal stem cells, which was statistically significant. Then, the neural differentiation of cytokine-inducted human mesenchymal stem cells suggested that transplantation of the cytokine-inducted human mesenchymal stem cells may provide the possibility of the clinical implication for cerebral stroke.

Author dc:creator

• 김, 형석

Contributors dc:contributor

• 조, 경기
• 대학원 의학과
• 199824359

• stroke
• focal cerebral ischemia
• middle cerebral artery occlusion
• stem cell transplantation
• cytokine-inducted human mesenchymal stem cells
• behavioral tests

Language dc:language

ko

Identifier

http://dcoll.ajou.ac.kr:9080/dcollection/jsp/common/DcLoOrgPer.jsp?sItemId=000000000769
000000000769

OAI identifier oai:identifier

oai:repository.ajou.ac.kr:201003/1514