Nrf2-Dependent Overexpression of Sulfiredoxin and Peroxiredoxin III in Human Lung Cancer, Especially Squamous Cell Carcinoma

"TABLE OF CONTENTS ABSTRACT i TABLE OF CONTENTS iii LIST OF FIGURES v ABBREVIATION vi Ⅰ. INTRODUCTION 1 Ⅱ. MATERIALS AND METHODS 6 A. Materials 6 B. Subjects 6 C. Cell culture and viability 7 D. Transfection and depletion of siRNA 7 E. Immunocytochemistry 8 F. Co-immunoprecipitation and immunoblot analysis 8 G. Two-dimensional electrophoresis 9 H. Subcellular fractionation 10 I. Immunohistochemistry 10 J. Statistical analysis 11 Ⅲ. RESULTS 12 A. Localization of Prx III and Srx in A549 cells 12 B. Oxidation of Prx III in mink lung epithelial cells 12 C. Srx dependent regulation of Prx III oxidation in A549 cells 15 D. Srx is translocated into the mitochondria under oxidative stress 15 E. Overexpression of Prx I and III isoforms in human lung cancer 19 F. Nrf2 dependent expression of ARE-proteins in human lung cancer 19 G. Overexpression of Srx in human lung cancer 19 H. Survival of patients with lung cancer based on Srx expression 26 I. Expression of Srx in human lung cancer tissue 26 Ⅳ. DISCUSSION 28 Ⅴ. CONCLUSION 30 REFFERENCE 32 국문요약 40 "

Author dc:creator

• 김, 영선

Contributors dc:contributor

• 황, 성철
• 대학원 의학과
• 104930

Language dc:language

en

Identifier

http://dcoll.ajou.ac.kr:9080/dcollection/jsp/common/DcLoOrgPer.jsp?sItemId=000000010432
000000010432

OAI identifier oai:identifier

oai:repository.ajou.ac.kr:201003/1451