Cell death and Pro-inflammatory Cytokine Release of Rat Microglial Cells by Naegleria fowleri

Naegleria fowleri, a free-living amoeba, causes fatal primary amoebic meningoencephalitis in humans and experimental animals. In previous study, N. fowleri trophozoites showed the highly cytopathic activity and cytotoxicity against rat microglial cells by the morphological observation and 51Cr release assay. In the present study, to determine whether a pathogenic N. fowleri lysate shows the cytopathic effects against primary cultured rat microglial cells, the morphological changes of microglial cells was observed by a light, scanning and transmission electron microscopes. And then, the cytotoxicity of N. fowleri lysate against rat microglial cells was also observed by (51)^Cr release assay. In addition, the pro-inflammatory cytokine release from microglial cells in co-culture system was estimated. As results with a light and electron microscopes, most of microglial cells were severely destroyed by N. fowleri lysate, showing the necrotic (above 85%) and apoptotic cell death (below 15%) in a time- and dose dependent manner. As the results of (51)^Cr release assay, the cytotoxicity of N. fowleri lysate against microglial cells were 14.6, 21.9, 38.5 and 71.5% at 3, 6, 12 and 24 h post incubation, respectively. And then, the amount of cytokines released from microglial cells in co-culture system at 3, 6 and 12 hr were 121.6, 90.4 and 81.0 pg/ml of TNF-α, 88.5, 92.7 and 190.1 pg/ml of IL-1β, and 298.8, 414.9 and 251.1 pg/ml of IL-6, respectively.

Author dc:creator

• 박, 창은

Contributors dc:contributor

• 신, 호준
• 대학원 의학과
• 200624502

• Naegleria fowleri
• primary amoebic meningoencephalitis
• microglial cells
• cytopathic effect
• cytotoxicity
• pro-inflammatory cytokine

Language dc:language

en

Identifier

http://dcoll.ajou.ac.kr:9080/dcollection/jsp/common/DcLoOrgPer.jsp?sItemId=000000009136
000000009136

OAI identifier oai:identifier

oai:repository.ajou.ac.kr:201003/1403