The Intracellular Changes and the Localization of Core particles by Hepatitis B Virus P Protein-Deficient Mutant

PURPOSE: Hepatitis B virus (HBV) core particles move to ER or recycle back to the nucleus. This moving mechanism is thought to be important in HBV replication and HBV life cycles. P protein-deficient mutant can form core particles in the absence of P protein and pregenomic RNA (pgRNA). In this study, the intracellular changes of P protein-deficient mutant expressing stable cells and the localization of P protein-deficient mutant core particles were observed. MATERIALS & METHODS: HBV replicating and non-replicating stable cells had been established in HuH7 hepatoma cell lines. Using Northern blot and Southern blot analyses, HBV transcription and replication were examined in HBV replicating and non-replicating stable cells. Distribution of intracellular organelles was examined by immunofluorescene assay. Mitochondria were stained using Mito-Tracker. Core particles were visualized using anti-HBc antibody. Microtubules, vimentin, nuclear pore complex (NPC) were visualized using mouse anti-tubulin, mouse anti-vimentin, and mouse anti-NPC antibody, respectively. RESULTS: P-deficient mutant core particles co-localized with microtubule, NPC, and vimentin, like HBV wild type (wt) core particles. Core particles of priming- and reverse transcriptase-deficient mutants that only consist of immature core particles, were not co-localized with microtubule, NPC, and vimentin. The mitochondria of P-deficient mutant expressing stable cells aggregated into peri-nuclear region like those of HBV wt expressing stable cells. CONCLUSION: These results indicated that P protein-deficient mutant core particles act like HBV wt core particles, suggesting that P protein-deficient mutant and mature core particles might share similar properties.

Author dc:creator

• 정, 재성

Contributors dc:contributor

• 김, 경민
• 대학원 의학과
• 105748

• Hepatitis B Virus
• P-protein deficient mutant
• immature core particle
• mature core particle
• B형 간염 바이러스

Language dc:language

en

Identifier

http://dcoll.ajou.ac.kr:9080/dcollection/jsp/common/DcLoOrgPer.jsp?sItemId=000000002068
000000002068

OAI identifier oai:identifier

oai:repository.ajou.ac.kr:201003/1329