Fas-associated factor 1 is involved in neurofibrillary tangle-mediated cell death of basal forebrain cholinergic neurons in P301L transgenic mice

PART I 1 Ⅰ. INTRODUCTION 2 A. Basal forebrain of mammals 2 B. Neuron types in the basal forebrain 2 C. Alzheimer’s disease 3 D. Normal functions of tau protein 4 E. Structure and Posttranslational Modifications of the Tau Protein 5 F. Distribution of tau phosphorylation sites and NFT formation in AD brains 7 G. Monoclonal antibody AT8 recognizes tau phosphorylation 11 H. Cholinergic dysfunction in the basal forebrain in AD cases 12 I. FTDP-17 13 J. Cholinergic dysfunction in P301L transgenic mice 14 Ⅱ. MATERIALS AND METHODS 16 1. Animals 16 2. Antibodies 16 3. Immunohistochemistry 16 4. Thioflavin-S staining 17 5. Sarkosyl fraction of Tau protein and immunoblotting 17 6. Stereological analysis 18 7. Statistical analysis 19 Ⅲ. RESULTS 20 Ⅳ. DISCUSSION 28 Ⅴ. CONCLUSION 30 PART II 31 ABSTRACT 32 Ⅰ. INTRODUCTION 34 A. Reelin expression in GABAergic neurons 34 B. Reelin structure 36 C. Reelin fragments 38 D. Reelin receptors and reelin signaling 39 E. The involvement of Reelin signaling in neuropsychiatric disorders 41 F. Astrogliosis 41 Ⅱ. MATERIALS AND METHODS 44 1. Animals 44 2. Brain section preparation and Immunohistochemistry 44 3. Stereological analysis 45 4. Western blot analysis 45 5. Statistical analysis 46 Ⅲ. RESULTS 47 Ⅳ. DISCUSSION 64 Ⅴ. CONCLUSION 66 CONCLUSION OF PART I, II 67 PART III 69 ABSTRACT 70 Ⅰ. INTRODUCTION 72 A. Mammalian neocortex 73 B. Reelin function in the developing cortex 73 C. Preplate and preplate splitting 75 Ⅱ. MATERIALS AND METHODS 77 1. Animals 77 2. Production of recombinant Reelin 77 3. Culture of neurospheres 78 4. Immunofluorescence 79 Ⅲ. RESULTS 81 Ⅳ. DISCUSSION 99 Ⅴ. CONCLUSION 103 REFERENCES 104 국문요약 116

Author dc:creator

• 김, 종무

Contributors dc:contributor

• 이, 영돈
• 대학원 의생명과학과
• 201125165

• P301L transgenic mice
• septal region
• tau phosphorylation
• Alzheimer's disease

Language dc:language

en

Identifier

http://dcoll.ajou.ac.kr:9080/dcollection/jsp/common/DcLoOrgPer.jsp?sItemId=000000021554
000000021554

OAI identifier oai:identifier

oai:repository.ajou.ac.kr:201003/13019