The role of autophagy in allergic inflammation: a new target for severe asthma

I.INTRODUCTION 1 II.MATERIALS AND METHODS 4 1. Mice 4 2. EthicsStatement 4 3. Sensitization and challenge 4 4. Treatment protocols 5 5. Airway resistance measurements 6 6. Bronchoalveolar lavage and lunghistology 6 7. Cytokine levels in lung BALfluid 7 8. The measurement ofLC3expression 8 9. Electron microscopy 8 10. Atg5 knockdown 8 11. Double immunofluorescence staining 10 12. Data analysis 10 III.RESULTS 12 1. The establishment of severe asthma model 12 2. The level ofLC3 expression in severe asthma model 15 3. Electron microscopy results 16 4. Inhibition of autophagy improved AHR and airway inflammation 17 5. Knockdown of Atg 5 attenuates AHR and airway inflammation 20 6. Positive correlation betweeneosinophilcounting and LC3 expression 23 7. Localization of autophagy in severe asthma model 25 8. The effect of asthma drugs on autophagic responses 30 9. Comparisons of treatment effects of asthma drugs 36 IV.DISCUSSION 38 V.CONCLUSION 43 REFERENCES 44 국문요약 49

Author dc:creator

• 유, 경남

Contributors dc:contributor

• 대학원 의학과
• 201025205

• Allergicasthma
• Autophagy
• Eosinophil
• Interleukin 5

Language dc:language

en

Identifier

http://dcoll.ajou.ac.kr:9080/dcollection/jsp/common/DcLoOrgPer.jsp?sItemId=000000020525
000000020525

OAI identifier oai:identifier

oai:repository.ajou.ac.kr:201003/11902