Global ETD Search
Search theses and dissertations gathered from participating repositories worldwide. Every result links back to the library that holds it. No account is needed.
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Showing 1 to 20 of 36 for “"ATG5"”.
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Structural and functional characterization of the autophagy proteins Atg5 and Atg16L1 and their interaction partners
… von Autophagosomvesikeln essentielle Atg12-Atg5~Atg16 Komplex assoziiert mit der wachsenden Isolationsmembran. Dieser Komplex oligomerisiert über die Coiled-Coil Domäne von Atg16 und diese Multimerisierung ist notwendig für das Wachstum der Isolationsmembran. In dieser Arbeit wurde das …
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The Functions of Autophagy Genes in Lymphocytes and Osteoclasts
… found that the essential autophagy gene <italic>Atg5</italic> was important for B cell development and for the maintenance of B-1a B cell numbers but not peripheral B-2 B cell numbers. In T cells, deletion of the essential autophagy genes <italic>Atg5</italic> or <italic>Atg7</italic> resulted in …
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Biochemical and Structural Characterization of the Atg8/LC3 Lipidation Pathway
… cascade including Atg7, Atg3, and Atg12–Atg5-Atg16 acting as E1, E2, and E3 enzymes, respectively. Atg7 initiates Atg8 conjugation by catalyzing their C-terminal adenylation and conjugation to the catalytic cysteine of Atg3. Ultimately, the Atg12–Atg5-Atg16 complex catalyzes Atg8 ligation …
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Paneth Cell-Dependent Intestinal Homeostasis During T. Gondii Infection
… autophagy via induction of IFN-g. Deletion of Atg5 in Paneth cells resulted in exaggerated intestinal inflammation characterized by the complete destruction of the intestinal crypts seen in pan-epithelial Atg5 deficient mice. An evaluation of Atg5 in intestinal organoids and in T. …
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Roles of Neutrophil Activation in Asthma
… controls (NC) to a genetic association study of ATG5 and ATG7 variants with neutrophilic airway inflammation. ATG5 (-769T>C, -335G>A, and 8830C>T) and ATG7 (-100A>G and 25108G>C) polymorphisms were genotyped using the SNaPshot method. The transcriptional activities of ATG5 -769T>C and -335G>A …
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Autophagy maintains the homeostatic environment in the male reproductive organs playing a key role in fertility
… short hairpin (shRNA) mouse model (called ATG5i) targeting Atg5, we created a mouse model that demonstrates ubiquitous inhibition of autophagy. With this model, we investigated the effects of autophagy inhibition and restoration in the environment of male reproductive organs using a …
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Autophagy mechanisms in sputum and peripheral blood cells of patients with severe asthma: a new therapeutic target
… hydroxychloroquine (HCQ) were used. Knockdown of ATG5 and Beclin-1 was performed in A549 cells, and the therapeutic effects of dexamethasone were evaluated. Results: Higher autophagy levels were noted in sputum granulocytes, PBCs, and PBEs from patients with severe asthma than from patients with …
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Scalable methods for the discovery of autophagy and disease genes
… and (2) an assay for levels of the ATG12-ATG5 conjugate, an important early autophagy marker. SRAI-LC3B can be used to assess autophagic flux via flow cytometry or microscopy and responds robustly to established pharmacological and genetic controls. Additionally, we exploit the optical …
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Autophagy and Inflammation During Rickettsial Infection
… targeted by autophagosomes. Using BMMs from Atg5flox/flox Lyz-Cre and Atg5flox/flox mice, I showed that rickettsia-induced autophagy is Atg5-dependent. Additionally, deletion of autophagy gene Atg5 in Atg5flox/flox Lyz-Cre BMMs significantly reduced bacterial replication compared to …
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Atm Signaling to Tsc2: Mechanisms and Implications For Cancer Therapy
… death. Using a genetic approach to knock-down Atg5 expression with shRNA in SKOV3 ovarian carcinoma cells, we compared the cytotoxicity of cisplatin in vector or Atg5 knock-down cells, and demonstrated that autophagy does not play any significant role in the response to cisplatin in this cell …
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Myc influences glutamine metabolism to induce autophagy in tumorigenesis
… regulators of autophagy, such as TOR, Atg1, Atg5 and ammonia, a by-product of glutamine catabolism, by dissecting these mechanisms both in normal epithelial clones and hyperproliferating RasV12 -expressing cells. Our results show that Myc promotes the transcription of glutamine-related genes …
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The role of autophagy in allergic inflammation: a new target for severe asthma
… ofLC3expression 8 9. Electron microscopy 8 10. Atg5 knockdown 8 11. Double immunofluorescence staining 10 12. Data analysis 10 III.RESULTS 12 1. The establishment of severe asthma model 12 2. The level ofLC3 expression in severe asthma model 15 3. Electron microscopy results 16 4. Inhibition of …
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A ROLE OF PRL-3 IN AUTOPHAGY AND ITS REGULATION IN HUMAN CANCERS
… AUTOPHAGOSOME FORMATION, AND ACCELERATES ATG5 DEPENDENT LC3 CONVERSION. PRL-3 OVEREXPRESSION ALSO ACCELERATES THE DEGRADATION OF SQSTM/P62, WHICH IS A KNOWN AUTOPHAGY SUBSTRATE. SURPRISINGLY, PRL-3 ITSELF IS ALSO DEGRADED BY AUTOPHAGY, FORMING A NEGATIVE FEEDBACK LOOP WITH AUTOPHAGY. …
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A Protective Role Of Autophagy In A Drosophila Model Of Friedreich's Ataxia (frda)
… increases autophagy activity. The comparable Atg5 mRNA level in both Frataxin deficiency and overexpression flies indicates this induction of autophagy in FRDA flies is Atg5 independent. Autophagy inducer Methylene blue and rapamycin could partially prolong the longevity and restore the …
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Mechanisms Underlying The Sensitivity and Resistance of Gastric Cancer Cells to Met Inhibitors
… obligate components of the autophagy pathway (ATG5 and ATG7) blocked cell death induced by crizotinib or RNAi-mediated knockdown of MET, and mechanistic studies localized the effects of autophagy to cytochrome c release from the mitochondria. Overall, the data reveal a novel relationship …
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Evaluation of the role of autophagy in ovarian cancer chemoresistance
… knockdown of the autophagy genes, BECN1 and ATG5, significantly reduced the recovery of OC cells that undergo autophagy following treatment with paclitaxel. In addition, cell cycle analysis demonstrated that OC cells can regain a ‘normal/parental’ DNA profile 7-days after drug treatment, …
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Epithelial Cell Autophagy in Antibacterial Defense of the Small Intestine
… in small intestinal epithelial cell autophagy (Atg5∆IEC), I have determined that epithelial cell autophagy is required to limit pathogen dissemination to extraintestinal sites. This study thus shows that autophagy is a critical mechanism of innate immune defense that protects intestinal …
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SHORT-CHAIN FATTY ACIDS INDUCED AUTOPHAGY SERVES AS AN ADAPTIVE STRATEGY FOR RETARDING MITOCHONDRIA-MEDIATED APOPTOTIC CELL DEATH
… or chloroquine) or genetically (knockdown of ATG5 or ATG7), the colon cancer cells became sensitized toward propionate induced apoptosis through activation of caspase 7 and its downstream effector caspase-3. The observations indicate that propionatetriggered autophagy serves as an adaptive …
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Characterisation of genetic complexity in chronic lymphocytic leukaemia
… overlapping regions (MOR) on del(6q) to the ATG5 and PRDM1 genes. Further autophagy related genes were also found to be present in other MORs. Analysis of the autophagy pathway at the protein level showed higher autophagy activity in primary CLL cells compared with age-matched controls. …
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A maternal low-protein diet during gestation induces hepatic autophagy gene expression only in the female offspring Sprague-Dawley rats
… including LC3b, P62/SQSTM1, Beclin1, Atg3, Atg7, Atg5, and Atg10 was increased significantly only in the female offspring by gestational low-protein diet. The induction of gene expression was consistent with the activated eIF2a/ATF4 pathway in female offspring. Furthermore, we found the maternal …
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