Ajou University
Identification of Peptidyl-prolyl cis/trans isomerases affecting Drosophila circadian rhythms
Abstract
dc:descriptionABSTRACT ⅰ TABLE OF CONTENTS ⅲ LIST OF FIGURES ⅴ LIST OF TABLES ⅵ Ⅰ. INTRODUCTION 1 Ⅱ. MATERIALS AND METHODS 5 A. PPIase genes in Drosophila melanogaster 5 B. Knockdown fly strains 7 C. Fly behavioral assays 7 D. Plasmids and methods for S2 cell-based assays 8 E. Immunoblotting 8 F. Immunoprecipitation 9 G. Immunohistochemistry 10 H. Quantitative real-time RT–PCR 11 Ⅲ. RESULTS 12 A. PPIases alters Drosophila circadian activity rhythms 12 B. DOD 19 1. Daily cycle of core clock protein in dod knockdown flies 19 2. Degradatin of PER is attenuated when DOD is overexpressed in S2 cells 22 3. DOD interacts with hyperphosphorylated PER in S2 cells 28 C. CG5808 30 1. Daily cycle of core clock protein in CG5808 knockdown flies 30 2. PER and TIM are altered in key brain pacemaker neurons when CG5808 is down-regulated 34 Ⅳ. DISCCUTION 37 Ⅴ. CONCLUTION 40 REFERENCES 41 국문요약 46
Author and committee
dc:creator, dc:contributor.*- Author dc:creator
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- 강, 소후
- Contributors dc:contributor
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- 김, 은영
- 대학원 의생명과학과
- 201324124
Rights
- Language dc:language
- en
Identifiers
dc:identifier.*- Identifier
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http://dcoll.ajou.ac.kr:9080/dcollection/jsp/common/DcLoOrgPer.jsp?sItemId=000000019503
000000019503 - OAI identifier oai:identifier
- oai:repository.ajou.ac.kr:201003/11830