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Showing 1 to 18 of 18 for “"PPIASE"”.
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NcCyP41, a two domain neurospora crassa cyclophilin : characterization of its peptidyl-prolyl cis/trans isomerase activity ; isolation and functional analysis of two novel NcCyP41-binding proteins
… then analysed by biochemical methods. <br> <br>PPIase activity of the entire protein and of its domains were tested in an in vitro assay. Catalytic efficiencies of NcCyP41 and its PPIase domain were nearly identical and inhibited by CsA. C-terminal TPR domain showed no PPIase activity. Thus, …
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Targeting Proteins of Aspergillus fumigatus for Monoclonal Antibody Production
… the host. In previous studies, our lab detected PPIase, Eglc, Ecm33, TrxR and CatB in IA mouse serum and urine, using an antigen discovery platform. In our current work, we seek to develop mAbs that specifically target PPIase, Eglc, Ecm33, TrxR and CatB as an effective diagnostic tool for the …
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Identification of Cyclophilin gene family in soybean and characterization of GmCYP1
… with peptidyl-prolyl cis-trans isomerase (PPIase) activity. PPIase catalyzes the interconversion of the cis- and trans-rotamers of the peptidyl-prolyl amide bond of peptides. After extensive data mining, I identified 62 different CYP genes in soybean (GmCYP1 to GmCYP62), of which 8 are …
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Zur Substratspezifität und Substratbindung des periplasmatischen Chaperons SurA aus <i>Escherichia coli</i>
… periplasmatische, als Peptidyl-Prolyl Isomerase (PPIase) und Chaperon charakterisierte Protein SurA aus Escherichia coli ist an der Reifung von trimeren Porinen der äußeren Membran beteiligt. Das Protein ist aus drei Regionen aufgebaut: der N-terminalen Region, zwei aufeinander-folgenden …
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Pin1: WW domain ligands, catalytic inhibitors, and the mechanism
The peptidyl prolyl cis/trans isomerase, PPIase, has been the focus of numerous studies in the field of cell cycle regulation since proline-directed phosphorylation is an essential signaling mechanism that might arrest cancer proliferation. Pin1 is the first phosphorylation-dependent PPIase enzyme …
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Identification of Peptidyl-prolyl cis/trans isomerases affecting Drosophila circadian rhythms
… Ⅰ. INTRODUCTION 1 Ⅱ. MATERIALS AND METHODS 5 A. PPIase genes in Drosophila melanogaster 5 B. Knockdown fly strains 7 C. Fly behavioral assays 7 D. Plasmids and methods for S2 cell-based assays 8 E. Immunoblotting 8 F. Immunoprecipitation 9 G. Immunohistochemistry 10 H. Quantitative real-time …
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Dictyostelium als Wirtsmodell und Funktionsanalyse des Virulenzfaktors Mip aus Legionella pneumophila
… Penetrationsfähigkeit konnte nach Hemmung der PPIase-Aktivität durch FK506 bzw. Rapamycin gehemmt werden. Ebenso waren Legionellen nach Hemmung der Serinproteaseaktivitäten im Transwell-System nicht mehr in der Lage die Barriere aus Epithelzellen mit extrazellulärer Matrix zu durchwandern. Mit …
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RAMIE (BOEHMERIA NIVEA): AN UNDERUTILIZED PLANT FOR A MULTIPURPOSE INDUSTRIAL APPLICATION; A PERENNIAL PLANT WITH PROTEIN IDENTIFICATION USING MALDI-MS, A BIOSORBENT FOR Hg (II) REMOVAL FROM WATER AND AS A BAST FIBER PRODUCTION VIA ENZYMATIC DEGUMMING
… study their benefits to the biological system. PPiase and RuBisCO with molecular weight 15 and 52 KDa were identified as the proteins after peptide mass fingerprinting analysis in mascot based based on their matched peptides. These proteins were known to contain some essential amino acids that …
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FKBP14 in Notch Signalling and Drosophila Development
… and present data establishing that both the PPIase and EF hand domains of FKBP14 are required for its function. Altogether, these data demonstrate that FKBP14 plays a critical role in development, one aspect of which includes regulating members of the Notch signalling pathway. Investigation …
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The Role of Cyp33 in MLL Mediated Gene Repression
… of HDAC1 and BMI1 to the RD of MLL. Also CYP33 PPIase activity was examined to determine if it is essential for HDAC1 and BMI1 binding to MLL.</p><p>Once HDAC1 and BMI1 were established to bind to MLL in a PPIase dependent manner, the chromatin modifications on the H3 histone tail, which are …
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Entwicklung eines GFP-Reportersystems in Legionella und molekularbiologische Funktionsanalyse des Legionella Mip-Proteins
… weiter nachzugehen. Da die in vivo Funktion von PPIasen (Peptidyl-Prolyl-cis/trans-Isomerasen) wie dem Mip-Protein in Prokaryoten bis heute weitgehend unbekannt ist, sollte im zweiten Teil dieser Arbeit versucht werden, einen Interaktionspartner zu identifizieren und den Einfluss der …
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Pin1 Catalytic and WW Domain Ligands
Pin1 is a peptidyl prolyl isomerase (PPIase) enzyme with two domains, the catalytic domain and the WW domain. Both domains specifically bind pSer/pThr–Pro motifs. Pin1 plays an important role in regulating the cell cycle, and it is involved in many diseases, such as cancer, HIV-1, Alzheimer's …
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Part 1 Design, Synthesis and Bioactivity of a Phosphorylated Prodrug for the Inhibition of Pin1; Part 2 Conformational Specificity of Cdc25c Substrate for Cdc2 Kinase using LC-MS/MS
The phosphorylation-dependent PPIase (peptidyl prolyl isomerase), Pin1 (Protein interacting with NIMA#1), has been found to regulate cell cycle through a simple conformational change, the cis-trans isomerization of phospho-Ser/Thr-Pro amide bonds. A variety of key cell cycle regulatory …
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Design, Syntheses, and Bioactivities of Conformationally Locked Pin1 Ground State Inhibitors
… preference of Pin1 for a cis amide bond in its PPIase domain. The A2780 ovarian cancer cell antiproliferation activity of these peptidomimetics parallels their respective Pin1 inhibition data. This research provides a start toward more drug-like Pin1 inhibitor design. Gly–<i>trans</i>–Pro …
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Die Funktion der Cochaperone FKBP51, FKBP52 und p23 bei der Signaltransduktion der Corticoidrezeptoren
… Wirkung benötigt. Im Gegensatz dazu liefert die PPIase-Aktivität von FKBP52 einen aktiven Beitrag für die Funktionalität der Corticoidrezeptoren, weil die analoge Mutation in FKBP52 zur einer Hemmung der Transaktivierung und nukleären Translokation des GRs und des MRs führt. Da diese Mutante …
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Isomerization-Locked Alkene Analogues of Xaa–Pro Dipeptides in the Proteins Collagen and Bora
… the prolyl amide is catalyzed by enzymes called PPIases. The PPIase Pin1 specifically catalyzes isomerization of the pSer–Pro sequence from the cis-conformation to the trans-conformation. Pin1 plays a crucial role in the G2→M transition of the cell cycle, implying the importance of cis-trans …
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NMR AS A TOOL TO QUANTITATIVELY CHARACTERIZE MOLECULAR MECHANISMS GOVERNING ACTIN DYNAMICS AND RICE LATERAL ROOT DEVELOPMENT
Protein-Protein interactions are fundamental for varied biological processes. These interactions are governed by different forces between the proteins, which result in a wide range of interaction strengths. Nuclear Magnetic Resonance (NMR) is an atomistic technique commonly used to understand …
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Pin1 Inhibitors: Towards Understanding the Enzymatic Mechanism
An important role of Pin1 is to catalyze the cis-trans isomerization of pSer/Thr-Pro bonds; as such, it plays an important role in many cellular events through the effects of conformational change on the function of its biological substrates, including Cdc25, c-Jun, and p53. The expression of Pin1 …